Skip to main content
. 2010 Aug 6;299(4):H1190–H1204. doi: 10.1152/ajpheart.01207.2009

Fig. 7.

Fig. 7.

Effect of ACh stimulation on coimmunoprecipitation of PGIS with Hsp90 under untreated (vehicle) or GA-treated conditions for PRAs from piglets raised in normoxia (A and C) or hypoxia (B and D) for either 3 days (A and B) or 10 days (C and D). PRA protein homogenates were immunoprecipitated with anti-Hsp90 (A, 3-day normoxic piglets, n = 8 vehicle-treated piglets and 8 GA-treated piglets; B, 3-day hypoxic piglets, n = 4 vehicle-treated piglets and 4 GA-treated piglets; C, 10-day normoxic piglets, n = 4 vehicle-treated piglets and 4 GA-treated piglets; and D, 10-day hypoxic piglets, n = 4 vehicle-treated piglets and 4 GA-treated piglets). The resultant immunoprecipitates were subjected to immunoblot analysis using antibodies against PGIS and Hsp90. The summary densitometric data show that ACh stimulation had no effect on the coimmunoprecipitation of PGIS with Hsp90 for untreated and GA-treated PRAs from any group of piglets.