Table 2.
6-Keto-PGF1α and TXB2 production in vehicle- and GA-treated PRAs under basal and AA-stimulated conditions for piglets exposed to control and hypoxic conditions for 3 and 10 days
| 3-Day Control | 3-Day Hypoxia | 10-Day Control | 10-Day Hypoxia | |
|---|---|---|---|---|
| 6-keto-PGFαproduction, pg/ml | ||||
| Number of PRAs/group | 7 | 7 | 8 | 6 |
| Vehicle-treated PRAs | ||||
| Basal | 30,963 ± 5,000 | 9,134 ± 1,531 | 6,396 ± 896 | 4,449 ± 896 |
| AA stimulation | 39,617 ± 7,120* | 17,766 ± 22,619* | 13,771 ± 1,193* | 10,295 ± 483* |
| GA-treated PRAs | ||||
| Basal | 29,922 ± 7,078 | 11,373 ± 1,854 | 7,480 ± 666 | 6,396 ± 896 |
| AA stimulation | 56,726 ± 13,315† | 20,971 ± 3,203† | 13,610 ± 1,456† | 13,771 ± 1,193† |
| TXB2production, pg/ml | ||||
| Number of PRAs/group | 7 | 7 | 8 | 6 |
| Vehicle-treated PRAs | ||||
| Basal | 3.7 ± 1.0 | 8.2 ± 2.8 | 1.0 ± 0.5 | 2.4 ± 0.8 |
| AA stimulation | 7.7 ± 1.6* | 12.0 ± 2.6* | 6.1 ± 1.9* | 8.3 ± 2.1* |
| GA-treated PRAs | ||||
| Basal | 3.6 ± 0.5 | 5.2 ± 1.0 | 1.3 ± 0.7 | 2.0 ± 1.0 |
| AA stimulation | 8.0 ± 1.6† | 12.0 ± 2.3† | 5.8 ± 2.2† | 7.5 ± 1.5† |
Values are means ± SD. AA, arachidonic acid.
*
Significantly different from vehicle-treated PRAs under basal conditions (P < 0.05 by paired t-test);
†
different from GA-treated PRAs under basal conditions (P < 0.05 by paired t-test).