Fig. 1.

GAPDH activity after S-nitrosocysteine (CySNO) and (Z)-1-{N-(3-ammoniopropyl)-N-[4-(3-aminopropylammonio)butyl]-amino}diazen-1ium-1,2-diolate (Sper/NO) treatment assayed immediately and after 1 and 3 h postincubation. Bovine aortic endothelial cells (BAECs) were incubated for 1 h with CysNO or Sper/NO in HBSS. Cells were washed and incubated further for up to 3 h in 2% FBS medium (1 and 3 h postincubation). GAPDH activity was determined in cell lysate following the consumption of NADH at 340 nm in the presence and absence of DTT. For the assay with DTT, cell lysate was pretreated with DTT for 30 min. Data were normalized to protein concentration and represent means ± SE (n = 3 experiments). A: CysNO treatment, assayed in the absence of DTT. B: CysNO treatment, assayed in the presence of DTT. C: Sper/NO treatment, assayed in the absence of DTT. D: Sper/NO treatment, assayed in the presence of DTT.