Fig. 3.

Intracellular S-nitrosothiols and GSH levels 1 and 3 h after CysNO removal. BAECs were incubated for 1 h with CysNO or Sper/NO in HBSS. Cells were washed and incubated further for up to 3 h in 2% FBS medium (0–3 h postincubation). A: intracellular S-nitrosothiol levels were measured by chemiluminescence. B: the GSH levels were determined in cell lysate by HPLC. Data were normalized to protein concentration and represent means ± SE (n = 3 experiments).