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Effects of CysNO and NO on purified GAPDH enzyme. GAPDH from chicken muscle was incubated for 30 min with indicated concentration of CysNO (A) or Sper/NO (B) in Tris buffer (pH 7.4). GAPDH activity was determined following the consumption of NADH at 340 nm in the presence and absence of DTT. For the assay with DTT, samples were pretreated with DTT for 30 min. Data represent means ± SE (n = 3 experiments).
