Fig. 6.

Inhibition of CysNO-treated GAPDH under turnover conditions. A: schemes of the treatment and the activity assay of GAPDH from chicken muscle. Arrows indicate in which order DTT and substrate were added. Numbers in parenthesis correspond to the activity of GAPDH measured under given conditions and presented in B. For the simplicity reasons, substrate addition on the scheme represents the addition of 3-phosphoglyceric phosphokinase as the GAPDH activity was measured in the coupled assay and 3-phosphoglyceric phosphokinase supplied the substrate for GAPDH. B: GAPDH from chicken muscle was incubated for 30 min with CysNO (400 μM) in Tris buffer (pH 7.4). DTT (5 mM) and substrate (3-phosphoglyceric phosphokinase) were added as indicated. Data represent means ± SE (n = 3 experiments).