Table 2.
Spot Number | Protein Name | Protein Description [Origin Species selected by MASCOT] |
Fold Change | p value | Mascot Score | Peptides Matched | Molecular Weight (Da) |
---|---|---|---|---|---|---|---|
1 | CpxR | DNA-Binding transcriptional regulator [Shigella flexneri 5 str. 8401] | +5.01 | 0.02136 | 185 | 11 | 26274 |
2 | RplE | 50 S ribosomal subunit protein L5 [Salmonella enterica serovar Typhi str. CT18] | +5.84 | 0.03998 | 85 | 8 | 20362 |
3 | RplF | 50 S ribosomal subunit protein L6 [Salmonella enterica serovar Typhi str. CT18] | +6.09 | 0.04065 | 177 | 7 | 18905 |
4 | SodA | Manganese superoxide dismutase [Escherichia coli O157:H7] | +7.51 | 0.01953 | 155 | 5 | 22886 |
5 | Dps* | starvation/stationary phase DNA protection protein [Salmonella enterica serovar Typhi str. CT18] | - | - | 482 | 12 | 18706 |
Table 2. Proteins in Table 2 are those with the highest and most statistically significant changes in protein expression following exposure to PA. Fold change is the level of change of each protein following PA adaptation. A Student's t test (performed by Melanie 5.0 gel analysis software) was used to determine the level of significance of expression values.
*As Dps was not detected by Melanie 5.0 in the unadapted control gels (for unknown reasons), no fold change or p value for this protein can be reported. This protein was selected for further study because of its prominence in PA adapted gels.