Fig. 4.
Influences of TNFα concentration and incubation time on the changes in Cap sensitivity in pulmonary sensory neurons. A: effect of increasing the TNFα concentration on Cap sensitivity in DiI-labeled nodose and jugular ganglion neurons. Changes in [Ca2+]i evoked by Cap challenge were measured in neurons after incubation with TNFα for ∼24 h. The number in each parenthesis represents the number of cells pretreated with a given concentration of TNFα. B: effect of TNFα (50 ng/ml) on the response of Δ[Ca2+]i evoked by Cap (100 nM) was altered by increasing incubation time in pulmonary sensory neurons. Different groups of neurons were used in the studies in A and B. #P < 0.05, significantly different from the response in corresponding control neurons (pretreated with vehicle, 0 ng/ml TNFα, for ∼24 h). *P < 0.05, significantly different from the response after TNFα incubation for 1 h. Data are means ± SE; when SE bars overlap between 2 adjacent data points, only 1 side is shown.