Figure 3. Comparison of NKG2D affinity for RAE-1 proteins.

HEK cell line was cotransfected with plasmids coding for EGFP as a transfection efficiency control and either RAE-1δ, RAE-1ε or RAE-1γ. After 3 days of culture, cells were incubated with increasing concentrations of NKG2D/Fc and PE-labeled anti-human IgG-Fc. Results were expressed as: (PE MFI measured on EGFP positive cells) - (PE MFI measured on EGFP negative cells).