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. 2010 Oct 19;5(10):e13466. doi: 10.1371/journal.pone.0013466

Figure 5. Localization of the rae-1δ transcriptional start site and activity of rae-1δ and rae-1ε promoters.

Figure 5

The 5′ RACE experiment was performed on enriched polyA+ RNA isolated from liver. A, The 5′ RACE product was sequenced and aligned with the rae-1δ genomic sequence. B, Detection in the liver and NOE cell line of Rae-1δ and Rae-1ε transcripts under the control of promoter 2 using a sense primer overlapping the exon 3b and the exon 4. C, NOE and C57sv cell lines were co-transfected with the luciferase reporter vector pGL3 Basic containing the different Rae-1 promoters or the pGL3-C vector containing the SV40 promoter and the pRL-null vector expressing renilla luciferase in order to assess the efficiency of transfection and to normalize the activity of the firefly luciferase. Luciferase activity was measured on each triplicate wells 48 h after transfection. The results are expressed as mean of 3 experiments in triplicate +/− SEM of firefly/renilla luciferase activity ratio for each condition. The assays of luciferase activity were analyzed with a MANOVA test (*: p<0.01 and **: p<0.001).