Table 1. Typing characteristics of the genotyping methods for the 60 isolates typed with all 3 methods.

	PFGE	MLVA9-UTRECHT	MLST
Typeability	91.7%	100%	100%
Costsa	€5.78	€7.21	€121.60
Timeb	5 days	2 days	7 days
Ease of interpretation	-	+	++
International comparisonc	-	+	++
Discriminatory Index	0.998 [0.995–1.0]	0.982 [0.968–0.998]	0.963 [0.936–0.991]

^a:cost per isolate tested, including materials, excluding labor and equipment depreciation since that is similar in all methods. MLST costs can be lower when not using outsourced sequencing.

^b: Time can be shorter with MLST. In this study we outsourced sequencing that took extra time.

^c: comparison with international data in database on http://pubmlst.org/paeruginosa/ for MLST and http://minisatellites.u-psud.fr/MLVAnet/ for MLVA.