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. 2010 Aug 18;104(4):2203–2213. doi: 10.1152/jn.01081.2009

Fig. 4.

Fig. 4.

Diffusion rates from the whole cell pipette to CA1 pyramidal neuron dendritic spines. A: live CA1 pyramidal cell visualized with Alexa 594 hydrazine (MW 520) introduced into the cell through a patch pipette. Box magnified to reveal dendrites and dendritic spines. B: single optical sections imaging Alexa 594 hydrazine and Oregon Green 488 dextran 3000. Top: dendrite (red) with Alexa 594 10 min following whole cell access. Bottom: same dendrite (green) with Oregon Green 488, (3000 MW dextran) again at 10 min after whole cell access. C: graph showing fluorescence rise of the 2 dyes at spines recorded in B plotted against time after whole cell access. Data are normalized (spine intensity/soma intensity). Red, Alexa 594; green, Oregon Green. Histograms show the relative intensity of Alexa 594 hydrazide (red), Oregon Green dextran (green) and fluorescently labeled albumin (MW 66,000; white) introduced in a further 3 neurons, between the soma and dendritic spines, 10 min after obtaining whole cell access.