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Patch-clamp recording of tracer XRITC-labeled parasympathetic cardiac motoneurons (PCMNs) in the nucleus ambiguus (NA) in brain stem slices. A: fluorescent imaging of PCMNs. ↓, the recorded PCMN in B. B: DIC imaging of single PCMNs for recording. ↓, a recorded PCMN. C: the merged image of A and B. Scale bar: 40 μm.
