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. 2010 Jul 28;104(4):2125–2138. doi: 10.1152/jn.00671.2009

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Fig. 11. — Effect of blockade of Ca²⁺ influx on spike frequency of PCMNs in control and NMDM. Cd²⁺ application abolished the difference in spike frequency of PCMNs in response to current injections between control and NMDM. A, 1 and 2: control; B, 1 and 2: NMDM. Representative traces of AP trains evoked by 1 s depolarizing current (100 pA) injection during control Artificial cerebrospinal fluid and after Cd²⁺ application in control and NMDM. C_. Before Cd²⁺, spike frequencies were significantly different between control and NMDM (†P < 0.05) which is consistent with Fig. 10C. After Cd²⁺ application, spike frequency significantly increased in both groups (†P < 0.05), with a significant greater increase in NMDM compared with control (*P < 0.05). There was no difference of spike frequencies between control and NMDM, confirming that the difference of spike frequencies between control and NMDM was Ca²⁺-dependent.