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Application of the broad muscarinic acetylcholine receptor (mAChR) agonist bethanechol (100 μm) inhibited the responses evoked by stimulation of trigeminal afferents by an average of 53.2 ± 5.5%, from −34.1 ± 9.2 to −15.9 ± 3.7 pA (n = 8, P < 0.05). A typical experiment is illustrated in A, with ▴ indicating evoked responses and summary results from 8 experiments in the bar graph (B) and scatterplot (C).
