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. Author manuscript; available in PMC: 2011 Oct 19.
Published in final edited form as: Cancer Cell. 2010 Oct 19;18(4):382–395. doi: 10.1016/j.ccr.2010.08.010

Figure 6. ProTAME-induced mitotic arrest is SAC-dependent.

Figure 6

(A) ProTAME-induced mitotic arrest is Mad2-dependent. HeLa H2B-GFP cells were transfected with indicated siRNAs between rounds of thymidine treatment. Following release, cells were treated with compounds and analyzed by time-lapse imaging. A graph of the same data with an expanded x-axis is shown in Figure S5A. (B) ProTAME rescues the mitotic defect induced by Mad2 knockdown. Asynchronous HeLa H2B-GFP cells were treated with Mad2 siRNA 24 h prior to addition of compound. Bar: 10 µm. (C) ProTAME-induced mitotic arrest is hesperadin-sensitive. Double thymidine synchronized HeLa H2B-GFP cells were treated with compounds 8 h following release. (D) UbcH10 or Cdc27 knockdown induces a hesperadin-sensitive mitotic delay. HeLa H2B-GFP cells were transfected with indicated siRNA between rounds of thymidine synchronization and treated with hesperadin 8 h following release. See also Figure S5 and Movies S5–6.