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. Author manuscript; available in PMC: 2010 Oct 20.
Published in final edited form as: Breast. 2007 Oct 24;17(2):172–179. doi: 10.1016/j.breast.2007.08.009

Fig. 5.

Fig. 5

Enhancing effects of E2 and iron on Ki67 and PCNA, markers of cell proliferation. MCF-7 cells grown in 0.1% FBS α-MEM were treated with E2 (10−9 M), iron (10 µM) or E2 + iron cultured in 6-well dish for 6 and 24 h. Cells were collected and lysed using M-Per lysis buffer. Thirty µg of protein samples were loaded to each well for Ki67, PCNA, and β-tubulin Western blotting. A representative gel was shown from three replicates (A) and folds of increase over controls were shown in (B) after quantification by densitometry. *Significantly different from control for both Ki67 and PCNA, p < 0.05.