Figure 4.

Effect of platelet gel supernatant on protein synthesis and proline transport in human fibroblast cultures. The cultured human fibroblasts were incubated for 72 hours in DMEM + 0.1% FBS (control) or in DMEM supplemented with 10% FBS or with platelet gel supernatant at the indicated concentrations. At the end of this period, L-leucine incorporation into the acid-insoluble fraction (panel A) and L-proline transport (panel B) were determined in parallel, as described in the Materials and Methods. The data are the means ± SD of three independent determinations.