Table 4.
Oxidation rate constant of gp120-OD8 GluC and Tryptic digests
| Peptide sequence [residue range] | Modified residues and its mass shift (Da) | SASA (Å2) in homology | Rate s-1 |
|---|---|---|---|
| RSTQLLLNGSLAEEE[1-15] | R1(+16/-43) L5/L6(+14/+16) L11(+14/16) E13(-30) and E14(-30) | L5 (0) L6(12.8) L11(49.7) E13(127.3) E14(132.4) | 7.7 ± 1.4 |
| IIIRSENITNNAK[16-28] | I16(+14/16) I18(+14/+16) R19(+16/-43) I23(+16) | I16(7.6) I18(21.6) R19(104.6) I23(24.5) | 3.7 ± 0.15 |
| TIIVQLNE [29:36] | I31(+14/16) | I31(19.8) | 3.9 ± 0.37 |
| INCTRPNNNTR[40-50] | I40(+16) C42(+32/16) R50 (+16) | I40(1.2) C42(4.0) R150(145.4) | 4.8 ± 1.6 |
| KSIHIGPGQAFYATGE[51:66](V3) | K51(+16) I53(+16) I55(+16) P57(+16) A60(+16) F61(+16) Y62(+16) H54(-23/-22/+16) | K51(134.9) I53(91.8) I55(129.9) P57(107.2) A60(43.6) F61(85.8) Y62(64.7) H54(81.7) | 14 ± 1.3 |
| SIHIGPGQAFYATGE[52:66] | I53(+14) I55(+16) A60(+16) F61(+16) P57(+16) Y62(+16) H54(-23/-22/+16) | I53(91.8) I55(129.9) P57(107.2) A60(43.6) F61(85.8) Y62(64.7) H54(81.7) | 7.8 ± 0.50 |
| SIHIGPGQAFYATGEIIGDIR [52:72] (V3) | I67or I68 (+16) I71 (+16/+14) R72(+16) | I67(137.8)or I68 (143.9) I71(98.9) R72(61.2) | 59 ± 8.6 |
| TKWNKTLQQVAK[81-92] | W83(+16/+32) K85(+16) | W83(5.1) K85(61.6) | 8.9 ± 0.61 |
| LREHFNNK[94-101] | H97(+16/-22) | H97(65.1) | 14± 1.9 |
| TIIFKPSSGGDLE[102-114] | I103(+16) F 105 (+16) K106(+16) P107(+16) L113(+16) | I103(4.4) F 105 (0) K106(87.0) P 107 (19.6) L113(91.4) | 2.2 ± 0.13 |
| ITTHSFNCGGE[115:125] | I115(+16) F120 (+16) C122 (+32) | I115(55.2) F120(16.8) C122 (21.3) | 2.0 ± 0.19 |
| GTYMFNGTR[143:151] | M146(+16) Y145(+16) F147(+16) R151(+16) | M146(198.0) Y145(152.7) F147(144.0) R151(190.6) | 28 ± 5.7 |
| TITLPCR[156:162] | I157(+16) L159 (+16) P160(+16)(or R162(+16)) | I157(37.9) L 159 (2.4) P160(73.3) R162(102.1) | 1.8± 0.39 |
| ITCKSNITGLLLTR[186:199] | C188(+48) K189 (+16) | C188(17.1) K189 (111.8) | 41± 2.5 |
| SNITGLLLTR[190:199] | I192(+16) L196(+16) L197(+16) | I192(0) L196(0) L197(0) | 0.20 ± 0.16 |
| QIINMWQGVGQAMYAPPIEGK[165:185] | M169 W170 V173 M177 Y178 P180 (+16) | M169(56.79) W170(127.27) V173(127.23) M177(116.98) Y178(89.4) P180(76.26) | 41± 6.4 |
| NKTETFRPGGGDMR [208-221] | P215(+16 ) M220(+16) R221(+16) | P215(0) M220(24.5) R221(156.0) | 41 ± 15 |