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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Dev Biol. 2010 Sep 21;347(2):301–314. doi: 10.1016/j.ydbio.2010.08.027

Figure 3.

Figure 3

Sacculation defects in epFoxM1 lungs. Embryos and newborn mice were treated with Dox from E7.5-18.5 (A-H) or E7.5-P1 (I-X), and lungs were harvested for histological and immunohistochemistry staining (IHC). (A-B) H&E staining showed sacculation defects in epFoxM1 lungs. (C-D) IHC showed FoxM1-ΔN protein in epithelium of epFoxM1 lungs. FoxM1-ΔN was not detected in control (WT) lungs (C). (E-F) pro-SPC expression was detected in epFoxM1 and control lungs. (G-H) FoxM1-ΔN (red) did not co-localize with pro-SPC (green). Erythrocytes showed autofluorescence (yellow) in alveolar regions. CCSP staining was detected in epFoxM1 and WT mouse lung airway epithelium (I-L). Immunofluorescent staining showed that FoxM1-ΔN did not co-express with FoxJ1 in bronchial epithelium (M-P). FoxM1-ΔN was found in pulmonary bronchiole (Br) of epFoxM1 mice where it was co-expressed with CCSP (Q-T). FoxM1-ΔN did not co-localize with CCSP in epithelial cells of abnormal pulmonary cysts (U-X).