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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Dev Biol. 2010 Sep 21;347(2):301–314. doi: 10.1016/j.ydbio.2010.08.027

Figure 9.

Figure 9

FoxM1-ΔN cooperates with activated K-Ras to induce lung tumor growth in vivo. TetO-FoxM1-ΔN mice were bred with SPC-rtTA/ TetO-K-Ras mice to generate SPC-rtTA/ TetO-FoxM1-ΔN/ TetO-K-Ras triple transgenic mice (epFoxM1/ epKras). Single transgenic littermates were used as controls. Mice were treated with Dox for 20 weeks. (A-H) Paraffin lung sections were stained with antibodies specific for the FoxM1-ΔN transgene (brown nuclei) and counterstained with nuclear fast red (red nuclei). Expression of FoxM1-ΔN alone did not cause lung tumors but resulted in formation of epithelial hyperplasia (arrows in D). (I) Tumor sizes in epFoxM1/ epKras mice were significantly increased compared to mice expressing K-Ras alone. Tumor areas in lung sections were measured by Axiovision Rel software. Ten random lung sections were used to determine statistical significance. A p value < 0.05 is shown with asterisk (*).