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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Environ Microbiol. 2010 May 7;12(9):2559–2586. doi: 10.1111/j.1462-2920.2010.02229.x

Fig. 2. Co-localization of the FCP of selected mutants with LAMP-1 in U937 macrophages.

Fig. 2

The cells were infected with each of the 125 mutants defective in intra-macrophage proliferation to determine co-localization of the phagosome with LAMP-1 at 6h post-infection. Bacteria were labeled with goat polyclonal antibody (green) and LAMP-1 was labeled with mouse monoclonal antibody (red). Representative confocal images of phagosomes harboring representative mutants from each functional group showing co-localization with Lamp-1 similar to the IglC mutant (A) or no co-localization, similar to the Sua5 WT-like phenotype (B). Data analyses were based on 100 infected cells analyzed from two different coverslips and the data were reproducible in two independent experiments.