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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Environ Microbiol. 2010 May 7;12(9):2559–2586. doi: 10.1111/j.1462-2920.2010.02229.x

Fig. 4. Representative mutants analyzed for their escape into the cytosol of U937 macrophages.

Fig. 4

U937 macrophages were infected with each of the 125 mutants of F. tularensis defective in intra-macrophage proliferation. Cytosolic bacteria were labeled with goat polyclonal antibody (red) loaded into the macrophage cytosol followed by permeabilization of all cellular membranes and labeling of all intracellular bacteria using mouse monoclonal antibody (green). Representative confocal images of F. tularensis mutant defective in phagosomal escape similar to the iglC mutant (A) and mutants that exhibit wild type-like Sua5 phenotype (B). Data analyses were based on 100 infected cells analyzed from two different coverslips and the data were reproducible in two independent experiments.