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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Environ Microbiol. 2010 May 7;12(9):2559–2586. doi: 10.1111/j.1462-2920.2010.02229.x

Fig. 5. Ultra-structural characterization of phagosomal escape.

Fig. 5

U937 macrophages were infected with F. tularensis for 1 h followed by 1 h of gentamicin treatment. After a total of 6 h, the infected cells were analyzed by TEM to determine whether bacteria were within intact or disrupted phagosomes. A) Six mutants were selected randomly to determine their phagosomal escape; B) Five mutants with discrepancy in the results of the fluorescence-based phagosome integrity assays and LAMP-1 co-localization were examined by TEM for their phagosomal escape.