Table 2.

List of growth defective or dissemination defective mutants identified in previous screens and are defective in both U937 macrophages and S2 cells

Strain Name	Locus Tag	Gene	Description
tnfn1_pw060323p03q172^α	FTN_0008		10 TMS drug/metabolite exporter protein
tnfn1_pw060420p02q151^β ^γ ^δ	FTN_0018	sdaC	serine permease
tnfn1_pw060323p02q177^β ^γ ^δ	FTN_0019	pyrB	aspartate carbamoyltransferase
tnfn1_pw060323p08q120^β ^γ ^δ	FTN_0020	carB	carbamoyl-phosphate synthase large chain
tnfn1_pw060510p02q160^β ^γ ^δ	FTN_0021	carA	carbamoyl-phosphate synthase small chain
tnfn1_pw060419p04q178^α	FTN_0028		conserved hypothetical membrane protein
tnfn1_pw060418p04q123^β	FTN_0070	pilE	Type IV pili, pilus assembly protein
tnfn1_pw060420p01q180^β ^γ ^δ ^π	FTN_0097		hydroxy/aromatic amino acid permease (HAAAP) family protein
tnfn1_pw060419p01q106^α	FTN_0111	ribH	riboflavin synthase beta-chain
tnfn1_pw060510p04q169^δ	FTN_0122	recA	recombinase A protein
tnfn1_pw060510p01q123^α	FTN_0132	lpsA	protein of unknown function
tnfn1_pw060323p03q125^α	FTN_0133		ribonuclease II family protein
tnfn1_pw060420p02q173^α	FTN_0169		conserved hypothetical membrane protein
tnfn1_pw060418p03q133^α	FTN_0199	cyoE	heme O synthase
tnfn1_pw060323p04q102^β ^γ ^δ	FTN_0211	pcp	pyrrolidone carboxylylate peptidase
tnfn1_pw060510p02q178^α	FTN_0217		L-lactate dehydrogenase
tnfn1_pw060420p04q134^α	FTN_0297		conserved protein of unknown function
tnfn1_pw060418p03q147^α	FTN_0299	putP	proline:Na+ symporter
tnfn1_pw060420p02q146^β ^γ ^δ	FTN_0300		glycosyl transferase, group 2
tnfn1_pw060328p01q167^β ^γ ^δ	FTN_0330	minD	septum formation inhibitor-activating ATPase
tnfn1_pw060323p08q146^δ	FTN_0331	minC	septum formation inhibitor
tnfn1_pw060328p08q156^α	FTN_0340		protein of unknown function
tnfn1_pw060323p06q113^β ^γ ^δ	FTN_0420	purCD	SAICAR synthetase/phosphoribosylamine-glycine ligase
tnfn1_pw060328p06q134^β ^γ ^δ	FTN_0443	maeA	NAD-dependent malic enzyme
tnfn1_pw060328p05q119^β ^γ ^δ ^π	FTN_0444		membrane protein of unknown function
tnfn1_pw060323p05q182^β ^γ ^δ	FTN_0504	cadC	lysine decarboxylase
tnfn1_pw060510p01q124^β ^γ ^δ	FTN_0507	gcvP1	glycine cleavage system P protein, subunit 1
tnfn1_pw060323p06q168^β ^γ ^δ	FTN_0545		glycosyl transferase, group 2
tnfn1_pw060419p02q135^β	FTN_0560	ksgA	dimethyladenosine transferase
tnfn1_pw060419p03q116^β ^γ ^δ	FTN_0593	sucD	succinyl-CoA synthetase, alpha subunit
tnfn1_pw060510p04q147^β ^γ ^δ	FTN_0599		protein of unknown function
tnfn1_pw060323p06q164^β ^γ ^δ	FTN_0624		serine permease
tnfn1_pw060418p02q128^δ	FTN_0633	katG	peroxidase/catalase
tnfn1_pw060420p01q168^α	FTN_0646	cscK	ROK family protein
tnfn1_pw060419p01q168^β ^γ ^δ	FTN_0651	cdd	cytidine deaminase
tnfn1_pw060510p04q168^δ	FTN_0666	uvrA	excinuclease ABC, subunit A
tnfn1_pw060328p04q123^β ^γ ^δ	FTN_0672	secA	preprotein translocase, subunit A (ATPase, RNA helicase)
tnfn1_pw060420p03q134^α	FTN_0710		type I restriction-modification system, subunit R (restriction)
tnfn1_pw060328p06q127^α	FTN_0713	ostA2	organic solvent tolerance protein OstA
tnfn1_pw060328p06q132^β ^γ ^δ	FTN_0728		predicted Co/Zn/Cd cation transporter
tnfn1_pw060323p06q115^α	FTN_0768	tspO	tryptophan-rich sensory protein
tnfn1_pw060323p06q105^α	FTN_0810		ROK family protein
tnfn1_pw060323p07q185^β ^γ ^δ	FTN_0814	bioF	8-amino-7-oxononanoate synthase
tnfn1_pw060418p01q141^β	FTN_0817		conserved protein of unknown function
tnfn1_pw060420p04q108^β ^γ ^δ ^π	FTN_0822		para-aminobenzoate synthase component I
tnfn1_pw060420p03q116^α	FTN_0824		major facilitator superfamily (MFS) transport protein
tnfn1_pw060328p01q128^α	FTN_0840	mdaB	NADPH-quinone reductase (modulator of drug activity B)
tnfn1_pw060420p04q176^β ^γ ^δ	FTN_0855		protein of unknown function
tnfn1_pw060420p02q175^α	FTN_0877	cls	cardiolipin synthetase
tnfn1_pw060323p04q104^α	FTN_0918		conserved protein of unknown function
tnfn1_pw060419p04q188^β ^γ ^δ	FTN_0925		protein of unknown function
tnfn1_pw060420p02q181^α	FTN_0933		protein of unknown function
tnfn1_pw060323p04q134^α	FTN_0938		hypothetical protein
tnfn1_pw060419p01q170^α	FTN_1006		transporter-associated protein, HlyC/CorC family
tnfn1_pw060323p08q141^α	FTN_1015		isochorismatase family protein
tnfn1_pw060418p01q153^α	FTN_1055	lon	DNA-binding, ATP-dependent protease La
tnfn1_pw060510p01q114^π	FTN_1073		DNA/RNA endonuclease G
tnfn1_pw060419p04q168^β ^γ ^δ	FTN_1091	aroA	3-phosphoshikimate 1-carboxyvinyltransferase
tnfn1_pw060328p08q188^α	FTN_1098		conserved hypothetical membrane protein
tnfn1_pw060328p02q109^α	FTN_1107	metlQ	methionine uptake transporter (MUT) family protein, membrane and periplasmic protein
tnfn1_pw060328p02q175^β ^γ ^δ	FTN_1131	putA	bifunctional proline dehydrogenase, pyrroline-5-carboxylate dehydrogenase
tnfn1_pw060418p03q107^β ^γ ^δ	FTN_1217		ATP-binding cassette (ABC) superfamily protein
tnfn1_pw060323p08q166^α	FTN_1232		conserved hypothetical membrane protein
tnfn1_pw060328p06q178^β ^γ ^δ	FTN_1241		DedA family protein
tnfn1_pw060510p03q135^β	FTN_1254		protein of unknown function
tnfn1_pw060420p04q196^β ^γ ^δ ^π	FTN_1256		membrane protein of unknown function
tnfn1_pw060323p03q102^β ^γ ^δ	FTN_1257		membrane protein of unknown function
tnfn1_pw060420p02q179^β ^γ ^δ	FTN_1263	comL	competence lipoprotein ComL
tnfn1_pw060418p01q149^β ^γ ^δ	FTN_1298		GTPase of unknown function
tnfn1_pw060328p01q144^β ^γ ^δ	FTN_1313		hypothetical protein
tnfn1_pw060328p06q163^β	FTN_1315		protein of unknown function
tnfn1_pw060510p01q110^α ^β ^γ ^δ	FTN_1321	iglD	intracellular growth locus protein D
tnfn1_pw060328p06q115^β ^γ ^δ	FTN_1322	iglC	intracellular growth locus protein C
tnfn1_pw060419p04q108^β ^γ ^δ	FTN_1325	pdpD	protein of unknown function
tnfn1_pw060510p01q142^β ^γ ^δ	FTN_1333	tktA	transketolase I
tnfn1_pw060418p01q191^α	FTN_1349		hypothetical protein
tnfn1_pw060418p01q185^α	FTN_1355		regulatory factor, Bvg accessory factor family
tnfn1_pw060418p02q109^π	FTN_1376		disulfide bond formation protein, DsbB family
tnfn1_pw060418p03q185^α	FTN_1400		S-adenosylmethionine-dependent methyltransferase
tnfn1_pw060419p04q135^α	FTN_1415		thioredoxin
tnfn1_pw060420p04q116^β ^γ ^δ	FTN_1421	wbtH	glutamine amidotransferase/asparagine synthase
tnfn1_pw060510p04q137^β ^γ ^δ	FTN_1425	wbtF	NAD dependent epimerase
tnfn1_pw060419p03q166^β ^γ ^δ ^π	FTN_1431	wbtA	dTDP-glucose 4,6-dehydratase
tnfn1_pw060418p02q122^β ^γ ^δ	FTN_1438		bifunctional protein: 3-hydroxacyl-CoA dehydrogenase/acyl-CoA-binding protein
tnfn1_pw060328p08q196^α	FTN_1459		short chain dehydrogenase
tnfn1_pw060323p06q110^β ^γ ^δ	FTN_1518	relA	GDP pyrophosphokinase/GTP pyrophosphokinase
tnfn1_pw060328p06q128^β	FTN_1530	lysA	diaminopimelate decarboxylase
tnfn1_pw060323p07q176^α	FTN_1534		conserved protein of unknown function
tnfn1_pw060418p02q178^α	FTN_1536		amino acid-polyamine-organocation (APC) superfamily protein
tnfn1_pw060418p01q150^γ	FTN_1586		sugar transporter, MFS superfamily
tnfn1_pw060510p01q146^β ^γ ^δ	FTN_1597	prfC	peptide chain release factor 3
tnfn1_pw060420p01q189^α	FTN_1611		major facilitator superfamily (MFS) transport protein
tnfn1_pw060323p04q160^β ^γ ^δ	FTN_1655	rluC	ribosomal large subunit pseudouridine synthase C
tnfn1_pw060419p04q180^δ	FTN_1682	frgA	siderophore biosynthesis protein
tnfn1_pw060323p03q163^β ^γ ^δ	FTN_1683		drug:H+ antiporter-1 (DHA1) family protein
tnfn1_pw060328p05q154^β ^γ ^δ	FTN_1777	trpG	anthranilate synthase component II

^α

(Kraemer et al., 2009)

^β

^γ

^δ

^π