Table 5.
Vacuolar vs. cytosolic localization of the mutants within U937 cells
| Strain Name | Locus Tag | Gene | %Co-localization with Lamp1 | Localization |
|---|---|---|---|---|
| Control | ||||
| Sua5/YciO/YrdC family protein | 24 | Cytoplasmic | ||
| Intracellular growth locus C | IglC | 81 | Phagosomal | |
| Proteins of unknown function | ||||
| tnfn1_pw060323p08q148 | FTN_0027 | 76 | Phagosomal | |
| tnfn1_pw060323p03q103 | FTN_0041 | 26 | Cytoplasmic | |
| tnfn1_pw060418p04q193 | FTN_0109 | 70 | Phagosomal | |
| tnfn1_pw060420p04q143 | FTN_0149 | 67 | Phagosomal | |
| tnfn1_pw060420p04q134 | FTN_0297 | 64 | Phagosomal | |
| tnfn1_pw060328p05q119 | FTN_0444 | 81 | Phagosomal | |
| tnfn1_pw060323p07q141 | FTN_0788 | 68 | Phagosomal* | |
| tnfn1_pw060420p04q176 | FTN_0855 | 89 | Phagosomal | |
| tnfn1_pw060420p02q178 | FTN_0915 | 70 | Phagosomal | |
| tnfn1_pw060419p04q188 | FTN_0925 | 72 | Phagosomal | |
| tnfn1_pw060323p03q147 | FTN_0930 | 55 | Phagosomal | |
| tnfn1_pw060420p02q181 | FTN_0933 | 66 | Phagosomal | |
| tnfn1_pw060510p01q108 | FTN_0977 | 69 | Phagosomal | |
| tnfn1_pw060420p01q127 | FTN_1175 | 80 | Phagosomal | |
| tnfn1_pw060420p04q196 | FTN_1256 | 83 | Phagosomal | |
| tnfn1_pw060323p01q113 | FTN_1343 | 60 | Phagosomal | |
| tnfn1_pw060328p02q110 | FTN_1457 | 32 | Cytoplasmic | |
| tnfn1_pw060420p01q132 | FTN_1624 | 70 | Phagosomal | |
| tnfn1_pw060420p02q184 | FTN_1696 | 45 | Cytoplasmic | |
| tnfn1_pw060328p06q155 | FTN_1764 | 76 | Phagosomal | |
| Hypothetical Proteins | ||||
| tnfn1_pw060323p03q142 | FTN_0030 | 63 | Phagosomal | |
| tnfn1_pw060328p06q180 | FTN_0038 | 79 | Phagosomal | |
| tnfn1_pw060420p02q173 | FTN_0169 | 32 | Cytoplasmic | |
| tnfn1_pw060323p01q181 | FTN_0336 | 40 | Cytoplasmic | |
| tnfn1_pw060328p05q136 | FTN_0384 | 28 | Cytoplasmic* | |
| tnfn1_pw060510p01q147 | FTN_0403 | 77 | Phagosomal | |
| tnfn1_pw060419p03q188 | FTN_0696 | 45 | Cytoplasmic* | |
| tnfn1_pw060323p01q163 | FTN_0727 | 68 | Phagosomal | |
| tnfn1_pw060419p02q102 | FTN_0792 | 80 | Phagosomal | |
| tnfn1_pw060510p02q108 | FTN_0847 | 64 | Phagosomal | |
| tnfn1_pw060323p07q105 | FTN_0895 | 30 | Cytoplasmic | |
| tnfn1_pw060510p03q192 | FTN_1098 | 75 | Phagosomal | |
| tnfn1_pw060418p01q191 | FTN_1349 | 56 | Phagosomal | |
| tnfn1_pw060328p06q182 | FTN_1395 | 80 | Phagosomal | |
| tnfn1_pw060328p04q136 | FTN_1406 | 84 | Phagosomal | |
| tnfn1_pw060328p02q129 | FTN_1612 | 69 | Phagosomal | |
| tnfn1_pw060420p02q176 | FTN_1686 | 89 | Phagosomal | |
| Metabolic Proteins | ||||
| tnfn1_pw060419p02q150 | FTN_0090 | 52 | Phagosomal | |
| tnfn1_pw060419p01q106 | FTN_0111 | ribH | 45 | Cytoplasmic |
| tnfn1_pw060328p06q174 | FTN_0125 | ackA | 80 | Phagosomal |
| tnfn1_pw060323p06q113 | FTN_0420 | 37 | Cytoplasmic | |
| tnfn1_pw060323p05q182 | FTN_0504 | 67 | Phagosomal | |
| tnfn1_pw060510p01q124 | FTN_0507 | gcvP1 | 28 | Cytoplasmic |
| tnfn1_pw060510p02q157 | FTN_0511 | 72 | Phagosomal | |
| tnfn1_pw060420p01q123 | FTN_0524 | asd | 64 | Phagosomal |
| tnfn1_pw060323p06q194 | FTN_0527 | thrC | 58 | Phagosomal |
| tnfn1_pw060510p03q168 | FTN_0598 | 42 | Cytoplasmic | |
| tnfn1_pw060328p06q130 | FTN_0692 | nadA | 68 | Phagosomal |
| tnfn1_pw060328p04q196 | FTN_0746 | alr | 72 | Phagosomal |
| tnfn1_pw060328p06q156 | FTN_0811 | birA | 78 | Phagosomal |
| tnfn1_pw060420p04q108 | FTN_0822 | 76 | Phagosomal | |
| tnfn1_pw060420p03q153 | FTN_0840 | mdaB | 81 | Phagosomal |
| tnfn1_pw060420p02q175 | FTN_0877 | cls | 70 | Phagosomal |
| tnfn1_pw060328p06q142 | FTN_0954 | 85 | Phagosomal | |
| tnfn1_pw060420p04q140 | FTN_0957 | 24 | Cytoplasmic | |
| tnfn1_pw060420p01q130 | FTN_0965 | 83 | Phagosomal | |
| tnfn1_pw060328p01q151 | FTN_0983 | 66 | Phagosomal | |
| tnfn1_pw060328p06q184 | FTN_1061 | 70 | Phagosomal | |
| tnfn1_pw060328p02q174 | FTN_1135 | aroB | 18 | Cytoplasmic |
| tnfn1_pw060328p03q107 | FTN_1222 | kpsF | 68 | Phagosomal |
| tnfn1_pw060420p04q194 | FTN_1231 | gloA | 87 | Phagosomal |
| tnfn1_pw060420p02q174 | FTN_1233 | 34 | Cytoplasmic | |
| tnfn1_pw060510p01q142 | FTN_1333 | tktA | 85 | Phagosomal |
| tnfn1_pw060418p02q109 | FTN_1376 | 78 | Phagosomal | |
| tnfn1_pw060328p06q150 | FTN_1494 | aceE | 87 | Phagosomal |
| tnfn1_pw060328p02q165 | FTN_1523 | 46 | Cytoplasmic | |
| tnfn1_pw060510p01q118 | FTN_1553 | nudH | 90 | Phagosomal |
| tnfn1_pw060420p04q105 | FTN_1584 | glpD | 23 | Cytoplasmic |
| tnfn1_pw060510p01q146 | FTN_1597 | prfC | 80 | Phagosomal |
| tnfn1_pw060419p02q112 | FTN_1619 | appC | 42 | Cytoplasmic* |
| tnfn1_pw060420p04q169 | FTN_1621 | 70 | Phagosomal | |
| tnfn1_pw060323p04q160 | FTN_1655 | rluC | 72 | Phagosomal |
| Transporter Proteins | ||||
| tnfn1_pw060420p04q149 | FTN_0008 | 35 | Cytoplasmic | |
| tnfn1_pw060418p04q168 | FTN_0141 | 25 | Cytoplasmic | |
| tnfn1_pw060323p03q141 | FTN_0619 | 26 | Cytoplasmic | |
| tnfn1_pw060323p06q164 | FTN_0624 | 83 | Phagosomal | |
| tnfn1_pw060328p06q132 | FTN_0728 | 65 | Phagosomal | |
| tnfn1_pw060323p01q177 | FTN_0799 | emrE | 34 | Cytoplasmic |
| tnfn1_pw060328p04q109 | FTN_0885 | 76 | Cytoplasmic | |
| tnfn1_pw060328p04q167 | FTN_0997 | 80 | Phagosomal | |
| tnfn1_pw060323p07q172 | FTN_1344 | 70 | Phagosomal | |
| tnfn1_pw060420p02q182 | FTN_1441 | 24 | Cytoplasmic* | |
| tnfn1_pw060420p01q189 | FTN_1611 | 87 | Phagosomal | |
| tnfn1_pw060510p01q152 | FTN_1711 | tyrP | 72 | Phagosomal |
| DNA Modification | ||||
| tnfn1_pw060510p02q141 | FTN_0133 | 81 | Phagosomal | |
| tnfn1_pw060323p03q122 | FTN_0577 | mutL | 31 | Cytoplasmic |
| tnfn1_pw060510p04q193 | FTN_0680 | uvrC | 60 | Phagosomal |
| tnfn1_pw060420p03q134 | FTN_0710 | 75 | Phagosomal | |
| tnfn1_pw060419p04q152 | FTN_1017 | 23 | Cytoplasmic | |
| tnfn1_pw060328p04q156 | FTN_1027 | ruvC | 60 | Phagosomal |
| tnfn1_pw060510p01q114 | FTN_1073 | 86 | Phagosomal | |
| tnfn1_pw060510p01q153 | FTN_1154 | 69 | Phagosomal | |
| tnfn1_pw060323p03q167 | FTN_1197 | recR | 27 | Cytoplasmic |
| Transferases | ||||
| tnfn1_pw060323p03q119 | FTN_0019 | pyrB | 63 | Phagosomal |
| tnfn1_pw060510p01q103 | FTN_0063 | ilvE | 68 | Phagosomal |
| tnfn1_pw060323p03q121 | FTN_0343 | 26 | Cytoplasmic | |
| tnfn1_pw060328p03q179 | FTN_0358 | 28 | Cytoplasmic | |
| tnfn1_pw060420p02q180 | FTN_0483 | 77 | Phagosomal | |
| tnfn1_pw060323p06q168 | FTN_0545 | 68 | Phagosomal | |
| tnfn1_pw060510p01q158 | FTN_0988 | prmA | 78 | Phagosomal |
| tnfn1_pw060510p02q144 | FTN_1234 | queA | 67 | Phagosomal |
| tnfn1_pw060418p04q172 | FTN_1418 | manC | 68 | Phagosomal |
| tnfn1_pw060510p01q119 | FTN_1428 | wbtO | 82 | Phagosomal |
| Transcription/Translation | ||||
| tnfn1_pw060510p03q168 | FTN_0598 | 42 | Cytoplasmic | |
| tnfn1_pw060419p04q129 | FTN_1290 | mglA | 71 | Phagosomal |
| Type IV Pilin | ||||
| tnfn1_pw060418p02q167 | FTN_1137 | pilQ | 66 | Phagosomal |
| tnfn1_pw060323p06q157 | FTN_1139 | pilO | 67 | Phagosomal |
| Others | ||||
| tnfn1_pw060323p08q110 | FTN_0286 | 73 | Phagosomal | |
| tnfn1_pw060420p01q168 | FTN_0646 | cscK | 77 | Phagosomal |
| tnfn1_pw060323p06q115 | FTN_0768 | tspO | 74 | Phagosomal |
| tnfn1_pw060328p06q167 | FTN_0985 | 37 | Cytoplasmic | |
| tnfn1_pw060419p02q137 | FTN_1034 | rnfB | 78 | Phagosomal |
| tnfn1_pw060420p02q177 | FTN_1145 | era | 84 | Phagosomal |
| tnfn1_pw060328p06q178 | FTN_1241 | 76 | Phagosomal | |
| tnfn1_pw060420p02q179 | FTN_1263 | comL | 79 | Phagosomal |
| tnfn1_pw060328p03q154 | FTN_1453 | 73 | Phagosomal | |
| tnfn1_pw060323p07q167 | FTN_1518 | relA | 81 | Phagosomal |
| Intergenic | ||||
| tnfn1_pw060323p03q164 | intergenic | 28 | Cytoplasmic | |
| tnfn1_pw060323p08q139 | intergenic | 80 | Phagosomal | |
| tnfn1_pw060328p06q190 | intergenic | 35 | Cytoplasmic | |
| tnfn1_pw060419p04q165 | intergenic | 65 | Phagosomal | |
| tnfn1_pw060419p04q189 | intergenic | 89 | Phagosomal | |
| tnfn1_pw060510p01q102 | intergenic | 90 | Phagosomal | |
| tnfn1_pw060510p01q112 | intergenic | 78 | Phagosomal | |
| tnfn1_pw060510p01q135 | intergenic | 87 | Phagosomal | |
*
Mutants with discrepancy between LAMP-1 co-localization and Phagosomal integrity assay.