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Disruption of remaining WT Pdcd2 allele in Pdcd2+/− ESCs by homologous recombination or selection for LOH in elevated G418.
| ESC line | Method | Colonies | Targeted/ LOH | Rate |
|---|---|---|---|---|
| Pdcd2+/− | HR | 48 | 6 | 12.5% |
| Pdcd2+/− | HR | 192 | 0 | 0 |
| Pdcd2+/− | G418 inc. | 96 | 0 | 0 |
| Flag-Pdcd2; Pdcd2+/− #5 | G418 inc. | 36 | 7 | 19.4% |
| Flag-Pdcd2; Pdcd2+/− #8 | G418 inc. | 24 | 19 | 79.2% |
| Flag-Pdcd2; Pdcd2+/− #8 | HR | 42 | 4 | 9.5% |
HR = homologous recombination. “inc.” = increase.
