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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Acta Biomater. 2010 Jul 1;6(12):4614–4621. doi: 10.1016/j.actbio.2010.06.033

Figure 4. Maintenance of ESC pluripotency on fibronectin microarrays in coculture with MEFs.

Figure 4

A. ESCs attached to the fibronectin microarray after 1 day. B. ESCs remained viable on the microarray after 2 days, based on robust calcein uptake (green). C. Phase-contrast images depict coculture of ESCs and MEFs on ECM microarray after 1 day. D. Alkaline phosphatase staining of ESCs in coculture with MEFs after 2 days indicates pluripotent phenotype. Scale bar: 500µm (A,C); 200µm (B); 300µm (D).