Table 3.
Metabolic genes required for intracellular proliferation of F. tularensis within S2 cells grouped according to metabolic pathways
| Amino acid metabolism | |||
|---|---|---|---|
| tnfn1_pw060323p08q120 | FTN_0020 | carB | carbamoyl-phosphate synthase large chain |
| tnfn1_pw060510p02q160 | FTN_0021 | carA | carbamoyl-phosphate synthase small chain |
| tnfn1_pw060328p05q159 | FTN_0118 | serine peptidase, S49 family | |
| tnfn1_pw060420p02q187 | FTN_0118 | serine peptidase, S49 family | |
| tnfn1_pw060328p06q174 | FTN_0125 | ackA | propionate kinase 2/acetate kinase A |
| tnfn1_pw060323p05q182 | FTN_0504 | lysine decarboxylase | |
| tnfn1_pw060510p01q124 | FTN_0507 | gcvP1 | glycine cleavage system P protein, subunit 1 |
| tnfn1_pw060510p02q154 | FTN_0511 | shikimate 5-dehydrogenase | |
| tnfn1_pw060510p02q157 | FTN_0511 | shikimate 5-dehydrogenase | |
| tnfn1_pw060510p04q157 | FTN_0511 | shikimate 5-dehydrogenase | |
| tnfn1_pw060323p06q194 | FTN_0527 | thrC | threonine synthase |
| tnfn1_pw060510p01q172 | FTN_0527 | thrC | threonine synthase |
| tnfn1_pw060510p03q172 | FTN_0527 | thrC | threonine synthase |
| tnfn1_pw060420p01q135 | FTN_0540 | pckA | phosphoenolpyruvate carboxykinase |
| tnfn1_pw060510p03q171 | FTN_0588 | asparaginase | |
| tnfn1_pw060418p04q167 | FTN_0935 | asnB | asparagine synthase |
| tnfn1_pw060328p06q142 | FTN_0954 | histidine acid phosphatase | |
| tnfn1_pw060328p02q175 | FTN_1131 | putA | bifunctional proline dehydrogenase, pyrroline-5- carboxylate dehydrogenase |
| tnfn1_pw060328p08q131 | FTN_1174 | murI | glutamate racemase |
| tnfn1_pw060328p06q128 | FTN_1530 | lysA | diaminopimelate decarboxylase |
| tnfn1_pw060328p05q101 | FTN_1532 | gdhA | glutamate dehydrogenase (NADP+) |
| tnfn1_pw060419p04q163 | FTN_1532 | gdhA | glutamate dehydrogenase (NADP+) |
| tnfn1_pw060328p05q160 | FTN_1729 | dapB | dihydrodipicolinate reductase |
| tnfn1_pw060510p01q178 | FTN_1729 | dapB | dihydrodipicolinate reductase |
| tnfn1_pw060328p04q104 | FTN_1730 | lysC | aspartate kinase III |
| tnfn1_pw060328p04q116 | FTN_9765 | choloylglycine hydrolase family protein | |
| Carbohydrate metabolism | |||
| tnfn1_pw060328p06q139 | FTN_0127 | gabD | succinate semialdehyde dehydrogenase (NAD(P)+ dependent) |
| tnfn1_pw060510p02q178 | FTN_0217 | L-lactate dehydrogenase | |
| tnfn1_pw060328p06q134 | FTN_0443 | maeA | NAD-dependent malic enzyme |
| tnfn1_pw060418p04q116 | FTN_0512 | glgX | pullulanase |
| tnfn1_pw060510p03q154 | FTN_0516 | glgA | glycogen synthase |
| tnfn1_pw060419p03q116 | FTN_0593 | sucD | succinyl-CoA synthetase, alpha subunit |
| tnfn1_pw060328p02q139 | FTN_0621 | eno | enolase (2-phosphoglycerate dehydratase) |
| tnfn1_pw060510p03q188 | FTN_0627 | chiA | chitinase, glycosyl hydrolase family 18 |
| tnfn1_pw060328p06q131 | FTN_0674 | glxK | glycerate kinase |
| tnfn1_pw060510p03q119 | FTN_0806 | glycosyl hydrolase family 3 | |
| tnfn1_pw060510p02q187 | FTN_1018 | aldolase/adducin class II family protein | |
| tnfn1_pw060328p03q107 | FTN_1222 | kpsF | phosphosugar isomerase |
| tnfn1_pw060328p05q128 | FTN_1329 | fbaA | fructose bisphosphate aldolase Class II |
| tnfn1_pw060510p04q137 | FTN_1425 | wbtF | NAD dependent epimerase |
| tnfn1_pw060419p03q166 | FTN_1431 | wbtA | dTDP-glucose 4,6-dehydratase |
| tnfn1_pw060323p07q169 | FTN_1438 | bifunctional protein: 3-hydroxacyl-CoA dehydrogenase/acyl-CoA-binding protein | |
| tnfn1_pw060418p02q122 | FTN_1438 | bifunctional protein: 3-hydroxacyl-CoA dehydrogenase/acyl-CoA-binding protein | |
| tnfn1_pw060328p06q150 | FTN_1494 | aceE | pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase |
| tnfn1_pw060420p04q105 | FTN_1584 | glpD | glycerol-3-phosphate dehydrogenase |
| tnfn1_pw060419p04q130 | FTN_1585 | glpK | glycerol kinase |
| tnfn1_pw060419p02q112 | FTN_1619 | appC | cytochrome bd-II terminal oxidase subunit I |
| tnfn1_pw060328p02q105 | FTN_1620 | appB | cytochrome bd-II terminal oxidase subunit II |
| tnfn1_pw060510p04q136 | FTN_1767 | rbsK | ribokinase, pfkB family |
| Nucleotide metabolism | |||
| tnfn1_pw060323p08q120 | FTN_0020 | carB | carbamoyl-phosphate synthase large chain |
| tnfn1_pw060510p02q160 | FTN_0021 | carA | carbamoyl-phosphate synthase small chain |
| tnfn1_pw060418p03q133 | FTN_0199 | cyoE | heme O synthase |
| tnfn1_pw060323p06q113 | FTN_0420 | SAICAR synthetase/phosphoribosylamine-glycine ligase | |
| tnfn1_pw060419p01q168 | FTN_0651 | cdd | cytidine deaminase |
| tnfn1_pw060328p04q151 | FTN_0661 | guaB | IMP dehydrogenase/GMP reductase |
| tnfn1_pw060510p01q159 | FTN_0695 | add | deoxyadenosine deaminase/adenosine deaminase |
| tnfn1_pw060328p01q151 | FTN_0983 | bifunctional protein: glutaredoxin 3/ribonucleotide reductase beta subunit | |
| tnfn1_pw060419p04q135 | FTN_1415 | thioredoxin | |
| tnfn1_pw060419p04q181 | FTN_1415 | thioredoxin | |
| tnfn1_pw060510p01q118 | FTN_1553 | nudH | dGTP pyrophosphohydrolase |
| Reductive Metabolism | |||
| tnfn1_pw060418p04q115 | FTN_0095 | nitroreductase | |
| tnfn1_pw060510p01q130 | FTN_0154 | rimK | glutathione synthase/ribosomal protein S6 modification enzyme |
| tnfn1_pw060419p04q153 | FTN_0597 | protein-disulfide isomerase | |
| tnfn1_pw060418p02q128 | FTN_0633 | katG | peroxidase/catalase |
| tnfn1_pw060418p02q142 | FTN_0826 | aldo/keto reductase family protein | |
| tnfn1_pw060328p01q128 | FTN_0840 | mdaB | NADPH-quinone reductase (modulator of drug activity B) |
| tnfn1_pw060420p03q153 | FTN_0840 | mdaB | NADPH-quinone reductase (modulator of drug activity B) |
| tnfn1_pw060323p05q141 | FTN_1033 | grxB | glutaredoxin 2 |
| tnfn1_pw060420p01q193 | FTN_1033 | grxB | glutaredoxin 2 |
| tnfn1_pw060420p04q194 | FTN_1231 | gloA | lactoylglutathione lyase |
| tnfn1_pw060510p02q164 | FTN_1231 | gloA | lactoylglutathione lyase |
| tnfn1_pw060510p04q146 | FTN_1231 | gloA | lactoylglutathione lyase |
| tnfn1_pw060328p08q196 | FTN_1459 | short chain dehydrogenase | |
| tnfn1_pw060418p01q131 | FTN_1557 | oxidoreductase iron/ascorbate family protein | |
| tnfn1_pw060418p04q112 | FTN_1621 | predicted NAD/FAD-dependent oxidoreductase | |
| tnfn1_pw060420p04q169 | FTN_1621 | predicted NAD/FAD-dependent oxidoreductase | |
| cholesterol Metabolism | |||
| tnfn1_pw060323p04q144 | FTN_0406 | sterol desaturase | |
| tnfn1_pw060418p01q189 | FTN_0406 | sterol desaturase | |
| Lipid Metabolism | |||
| tnfn1_pw060328p04q175 | FTN_0818 | lipase/esterase | |
| tnfn1_pw060420p02q175 | FTN_0877 | cls | cardiolipin synthetase |
| tnfn1_pw060328p06q166 | FTN_1273 | long chain fatty acid CoA ligase | |
| conenzyme synthesis | |||
| tnfn1_pw060328p06q130 | FTN_0692 | nadA | quinolinate sythetase A |
| tnfn1_pw060419p04q164 | FTN_0692 | nadA | quinolinate sythetase A |
| tnfn1_pw060420p01q148 | FTN_0694 | nadB | L-aspartate oxidase |
| tnfn1_pw060328p06q156 | FTN_0811 | birA | biotin--acetyl-CoA-carboxylase ligase |
| tnfn1_pw060323p07q185 | FTN_0814 | bioF | 8-amino-7-oxononanoate synthase |
| tnfn1_pw060419p02q138 | FTN_0814 | bioF | 8-amino-7-oxononanoate synthase |
| tnfn1_pw060418p01q124 | FTN_1245 | iscS | cysteine desulfurase |
| tnfn1_pw060419p03q126 | FTN_1278 | nadE | NAD synthase |
| tnfn1_pw060323p04q110 | FTN_1678 | nuoC | NADH dehydrogenase I, C subunit |
| tnfn1_pw060419p01q106 | FTN_0111 | ribH | riboflavin synthase beta-chain |
| tnfn1_pw060420p02q191 | FTN_0113 | ribC | riboflavin synthase alpha chain |
| Peptidoglycan biosynthesis | |||
| tnfn1_pw060328p06q125 | FTN_0496 | slt | soluble lytic murein transglycosylase |
| tnfn1_pw060323p04q102 | FTN_0211 | pcp | pyrrolidone carboxylylate peptidase |
| tnfn1_pw060418p03q177 | FTN_0211 | pcp | pyrrolidone carboxylylate peptidase |
| Aromatic compound biosynthesis | |||
| tnfn1_pw060323p08q141 | FTN_1015 | isochorismatase family protein | |
| tnfn1_pw060420p01q129 | FTN_1015 | isochorismatase family protein | |
| tnfn1_pw060328p02q174 | FTN_1135 | aroB | 3-dehydroquinate synthetase |
| ppGpp biosynthesis | |||
| tnfn1_pw060323p06q110 | FTN_1518 | relA | GDP pyrophosphokinase/GTP pyrophosphokinase |
| tnfn1_pw060323p07q167 | FTN_1518 | relA | GDP pyrophosphokinase/GTP pyrophosphokinase |