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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Environ Microbiol. 2010 May 7;12(9):2587–2612. doi: 10.1111/j.1462-2920.2010.02230.x

Table 4.

List of F. tularensis mutants defective in growth and lethality to D. melanogaster grouped according to function.

Strain Name Locus Tag Gene Description Log Reduction in Growth Relative to WT Percent survival Mut/WT cfu ratio lls
S2 U937
Controls
Wild type strain WT
Intracellular growth locus IglC
Cell Division
tnfn1_pw060328p01q167 FTN_0330 minD septum formation inhibitor-activating ATPase 2 2 13% 24.6E-03
DNA Modification
tnfn1_pw060510p01q114 FTN_1073 DNA/RNA endonuclease G 6 5 90% 1.2E-02
tnfn1_pw060328p04q156 FTN_1027 ruvC holliday junction endodeoxyribonuclease 4 3 60% 4.7E-01
tnfn1_pw060328p08q179 FTN_1491 adenine specific DNA methylase 2 50% 6.5E-01
tnfn1_pw060323p03q167 FTN_1197 recR RecFOR complex, RecR component 4 2 44% 6.7E-04
tnfn1_pw060328p06q158 FTN_1293 rnhB ribonuclease HII 5 3 40% 1.4E+00
tnfn1_pw060328p05q121 FTN_1487 restriction endonuclease 1 1 40% 9.0E+00
tnfn1_pw060510p04q193 FTN_0680 uvrC excinuclease ABC, subunit C 3 6 20% 1.0E-01
tnfn1_pw060510p01q153 FTN_1154 type I restriction-modification system, subunit S 6 5 20% 3.2E-01
tnfn1_pw060323p03q122 FTN_0577 mutL DNA mismatch repair enzyme with ATPase activity 5 5 0% 2.5E-02
FPI
tnfn1_pw060418p04q106 FTN_1319 pdpC hypothetical protein 5 3 90% 1.2E-04
tnfn1_pw060510p01q110 FTN_1321 iglD intracellular growth locus protein D 6 4 60% 2.1E-01
tnfn1_pw060328p06q115 FTN_1322 iglC intracellular growth locus protein C 5 33%
Hypothetical Protein
tnfn1_pw060420p04q159 FTN_1223 conserved hypothetical membrane protein 7 90% 3.1E-02
tnfn1_pw060419p03q188 FTN_0696 hypothetical membrane protein 2 2 78% 2.1E-02
tnfn1_pw060323p07q129 FTN_0759 conserved hypothetical protein 2 4 71% 2.1E+00
tnfn1_pw060328p06q180 FTN_0038 hypothetical protein 4 4 70% 3.5E-04
tnfn1_pw060323p03q142 FTN_0030 hypothetical membrane protein 3 4 63% 2.2E-03
tnfn1_pw060328p05q136 FTN_0384 conserved hypothetical protein 7 4 62% 2.3E-02
tnfn1_pw060328p06q126 FTN_0732 hypothetical protein 5 60% 1.6E+01
tnfn1_pw060328p02q129 FTN_1612 hypothetical protein 2 2 50% 2.3E-02
tnfn1_pw060328p04q136 FTN_1406 conserved hypothetical membrane protein 3 5 50% 9.0E-02
tnfn1_pw060328p08q101 FTN_0054 hypothetical protein 1 1 40% 3.1E-07
tnfn1_pw060418p01q191 FTN_1349 hypothetical protein 6 6 40% 2.0E-03
tnfn1_pw060323p07q105 FTN_0895 hypothetical protein 2 4 38% 4.4E-02
tnfn1_pw060420p02q139 FTN_0013 hypothetical protein 3 33% 2.3E+02
tnfn1_pw060418p02q175 FTN_0717 conserved hypothetical membrane protein 5 30% 2.3E-02
tnfn1_pw060420p02q173 FTN_0169 conserved hypothetical membrane protein 6 6 30% 1.9E+03
tnfn1_pw060323p06q178 FTN_1389 conserved hypothetical membrane protein 3 25% 9.6E-05
tnfn1_pw060419p04q117 FTN_1156 hypothetical protein 4 4 25% 3.0E+01
tnfn1_pw060419p02q102 FTN_0792 hypothetical protein 6 5 22% 2.3E+01
tnfn1_pw060419p03q187 FTN_1123 conserved hypothetical protein 3 20% 1.0E-01
tnfn1_pw060420p04q104 FTN_0466 conserved hypothetical protein 4 20% 1.3E+00
tnfn1_pw060328p06q185 FTN_0709 hypothetical protein 7 3 20% 1.3E+00
tnfn1_pw060510p04q192 FTN_1098 conserved hypothetical membrane protein 6 7 11% 2.7E-02
tnfn1_pw060510p01q184 FTN_1299 hypothetical protein 5 10% 1.3E-01
tnfn1_pw060418p03q151 FTN_0212 hypothetical membrane protein 3 10% 3.3E+00
Intergenic
tnfn1_pw060328p06q190 intergenic 3 3 89% 9.9E-03
tnfn1_pw060420p03q148 intergenic 5 80% 5.0E-02
tnfn1_pw060323p08q139 intergenic 4 4 44% 1.6E-02
tnfn1_pw060419p03q131 intergenic 2 2 40% 5.5E-01
tnfn1_pw060419p04q189 intergenic 3 5 22% 9.8E-02
Metabolic
tnfn1_pw060420p04q105 FTN_1584 glpD glycerol-3-phosphate dehydrogenase 5 3 100% 5.6E-04
tnfn1_pw060323p05q182 FTN_0504 lysine decarboxylase 4 4 100% 6.7E-04
tnfn1_pw060510p03q154 FTN_0516 glgA glycogen synthase 4 90% 3.6E-04
tnfn1_pw060328p06q142 FTN_0954 histidine acid phosphatase 4 4 90% 9.8E-03
tnfn1_pw060328p06q130 FTN_0692 nadA quinolinate sythetase A 2 3 90% 2.3E-01
tnfn1_pw060328p02q174 FTN_1135 aroB 3-dehydroquinate synthetase 4 3 80% 3.5E-04
tnfn1_pw060328p06q150 FTN_1494 aceE pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase 1 4 70% 4.7E-01
tnfn1_pw060418p02q109 FTN_1376 disulfide bond formation protein, DsbB family 4 4 70% 6.1E+02
tnfn1_pw060420p04q169 FTN_1621 predicted NAD/FAD-dependent oxidoreductase 4 4 67% 4.2E-01
tnfn1_pw060510p01q124 FTN_0507 gcvP1 glycine cleavage system P protein, subunit 1 7 5 67% 4.0E+00
tnfn1_pw060323p06q106 FTN_1552 acid phosphatase, PAP2 family 4 60% 2.8E-04
tnfn1_pw060419p01q106 FTN_0111 ribH riboflavin synthase beta-chain 5 4 60% 5.6E-03
tnfn1_pw060328p08q131 FTN_1174 murI glutamate racemase 5 60% 2.3E-01
tnfn1_pw060419p03q116 FTN_0593 sucD succinyl-CoA synthetase, alpha subunit 2 2 60% 2.6E+01
tnfn1_pw060323p04q110 FTN_1678 nuoC NADH dehydrogenase I, C subunit 2 55% 7.9E-04
tnfn1_pw060323p03q127 FTN_0567 tRNA synthetase class II (D, K and N) 2 6 55% 5.6E-03
tnfn1_pw060510p03q171 FTN_0588 asparaginase 2 1 50% 3.0E-02
tnfn1_pw060510p03q188 FTN_0627 chiA chitinase, glycosyl hydrolase family 18 2 50% 1.4E+00
tnfn1_pw060510p01q142 FTN_1333 tktA transketolase I 5 5 45% 1.8E-06
tnfn1_pw060510p02q178 FTN_0217 L-lactate dehydrogenase 2 44% 4.1E+01
tnfn1_pw060323p06q195 FTN_1390 Zn-dependent hydrolase 3 40% 1.2E-01
tnfn1_pw060419p01q168 FTN_0651 cdd cytidine deaminase 6 38% 3.5E+00
tnfn1_pw060510p02q187 FTN_1018 aldolase/adducin class II family protein 3 3 33% 3.0E-03
tnfn1_pw060328p02q105 FTN_1620 appB cytochrome bd-II terminal oxidase subunit II 3 6 33% 4.4E-02
tnfn1_pw060323p06q103 FTN_0711 predicted metal-dependent hydrolase 4 30% 2.4E-04
tnfn1_pw060328p08q145 FTN_0907 D-alanyl-D-alanine carboxypeptidase 3 30% 1.7E-01
tnfn1_pw060419p04q130 FTN_1585 glpK glycerol kinase 3 3 29% 4.4E-01
tnfn1_pw060328p01q151 FTN_0983 bifunctional protein: glutaredoxin 3/ribonucleotide reductase beta subunit 3 5 25% 1.9E-03
tnfn1_pw060419p02q112 FTN_1619 appC cytochrome bd-II terminal oxidase subunit I 7 5 22% 4.9E-02
tnfn1_pw060323p07q167 FTN_1518 relA GDP pyrophosphokinase/GTP pyrophosphokinase 4 4 22% 3.6E-01
tnfn1_pw060420p02q175 FTN_0877 cls cardiolipin synthetase 5 7 20% 6.8E-02
tnfn1_pw060328p03q174 FTN_1768 pepN aminopeptidase N 2 20% 8.0E+00
tnfn1_pw060418p02q122 FTN_1438 bifunctional protein: 3-hydroxacyl- CoA dehydrogenase/acyl-CoA- binding protein 4 20% 8.8E+01
tnfn1_pw060323p06q113 FTN_0420 SAICAR synthetase/phosphoribosylamine- glycine ligase 5 7 13% 2.0E-01
tnfn1_pw060510p01q146 FTN_1597 prfC peptide chain release factor 3 5 5 13% 6.8E+02
tnfn1_pw060510p01q118 FTN_1553 nudH dGTP pyrophosphohydrolase 5 5 11% 1.3E-01
tnfn1_pw060328p06q156 FTN_0811 birA biotin--acetyl-CoA-carboxylase ligase 7 6 11% 4.5E-02
tnfn1_pw060328p08q120 FTN_0987 tRNA-dihydrouridine synthase 4 11% 2.7E+00
tnfn1_pw060420p03q153 FTN_0840 mdaB NADPH-quinone reductase (modulator of drug activity B) 5 5 10% 2.9E-01
tnfn1_pw060420p01q130 FTN_0965 metal-dependent exopeptidase 3 3 9% 1.2E+00
tnfn1_pw060419p03q164 FTN_1186 pepO M13 family metallopeptidase 2 0% 4.0E-01
Others
tnfn1_pw060418p02q123 FTN_0107 lepA GTP-binding protein LepA 4 4 90% 9.4E-02
tnfn1_pw060323p06q105 FTN_0810 ROK family protein 4 70% 2.4E-04
tnfn1_pw060328p08q114 FTN_0266 htpG chaperone Hsp90, heat shock protein HtpG 2 67% 5.8E-01
tnfn1_pw060419p01q120 FTN_1488 prophage maintenance system killer protein (DOC) 6 60% 7.2E-01
tnfn1_pw060328p06q178 FTN_1241 DedA family protein 5 4 55% 2.8E-04
tnfn1_pw060418p04q181 FTN_0338 MutT/nudix family protein 2 1 50% 1.3E-01
tnfn1_pw060420p03q193 FTN_0768 tspO tryptophan-rich sensory protein 3 3 50% 1.4E-01
tnfn1_pw060419p02q137 FTN_1034 rnfB iron-sulfur cluster-binding protein 3 2 50% 1.2E+00
tnfn1_pw060323p03q111 FTN_0465 Sua5/YciO/YrdC family protein 2 2 33% 2.8E+00
tnfn1_pw060328p06q167 FTN_0985 DJ-1/PfpI family protein 6 2 20% 5.2E-01
tnfn1_pw060420p03q121 FTN_1064 PhoH family protein, putative ATPase 4 1 11% 1.2E-01
tnfn1_pw060328p03q154 FTN_1453 two-component regulator, sensor histidine kinase 2 2 11% 1.0E-02
tnfn1_pw060420p04q127 FTN_1031 ftnA ferric iron binding protein, ferritin- like 6 2 0% 3.0E+00
Proteins of unknown function
tnfn1_pw060420p01q142 FTN_0052 protein of unknown function 2 100% 1.6E-04
tnfn1_pw060420p04q176 FTN_0855 protein of unknown function 2 5 100% 6.9E-01
tnfn1_pw060323p06q122 FTN_0077 protein of unknown function 2 91% 3.2E-05
tnfn1_pw060418p02q186 FTN_1448 protein of unknown function 6 6 90% 8.8E-01
tnfn1_pw060418p03q108 FTN_0900 protein of unknown function with predicted hydrolase and phosphorylase activity 6 78% 1.3E-03
tnfn1_pw060418p04q193 FTN_0109 protein of unknown function 4 70% 2.9E-01
tnfn1_pw060328p06q173 FTN_0599 protein of unknown function 2 67% 3.3E-03
tnfn1_pw060328p08q104 FTN_0051 conserved protein of unknown function 3 67% 2.4E-02
tnfn1_pw060328p08q149 FTN_0439 protein of unknown function 4 60% 1.2E-03
tnfn1_pw060328p01q172 FTN_1542 conserved protein of unknown function 2 2 60% 5.5E-03
tnfn1_pw060323p05q127 FTN_0791 protein of unknown function 1 60% 8.0E-03
tnfn1_pw060418p04q121 FTN_1261 protein of unknown function 2 60% 7.7E-01
tnfn1_pw060418p02q157 FTN_1170 conserved protein of unknown function 6 5 60% 2.6E+01
tnfn1_pw060418p04q117 FTN_0207 protein of unknown function containing a von Willebrand factor type A (vWA) domain 2 56% 5.0E+00
tnfn1_pw060510p04q111 FTN_0861 conserved protein of unknown function 4 55% 4.2E+02
tnfn1_pw060323p04q183 FTN_1386 protein of unknown function 3 50% 1.2E-03
tnfn1_pw060418p01q142 FTN_0482 protein of unknown function 6 50% 4.8E-03
tnfn1_pw060328p05q124 FTN_1372 protein of unknown function 5 50% 1.5E+00
tnfn1_pw060323p07q176 FTN_1534 conserved protein of unknown function 3 50% 2.7E+00
tnfn1_pw060418p02q105 FTN_1343 conserved protein of unknown function 4 4 50% 6.9E+00
tnfn1_pw060323p03q157 FTN_0364 conserved protein of unknown function 2 45% 6.2E-01
tnfn1_pw060328p06q155 FTN_1764 protein of unknown function 7 6 40% 3.5E-06
tnfn1_pw060323p08q148 FTN_0027 conserved protein of unknown function 6 4 40% 2.7E-02
tnfn1_pw060418p02q145 FTN_1020 conserved protein of unknown function 5 40% 6.0E+00
tnfn1_pw060420p02q158 FTN_1071 protein of unknown function 5 33% 2.6E+02
tnfn1_pw060328p08q107 FTN_1774 protein of unknown function 3 33% 3.6E-01
tnfn1_pw060419p04q110 FTN_0048 conserved protein of unknown function 2 2 29% 4.2E+01
tnfn1_pw060419p04q188 FTN_0925 protein of unknown function 5 22% 5.4E+00
tnfn1_pw060323p07q141 FTN_0788 conserved protein of unknown function 5 5 22% 5.4E+00
tnfn1_pw060328p02q110 FTN_1457 protein of unknown function 5 5 20% 1.5E+00
tnfn1_pw060420p04q143 FTN_0149 conserved protein of unknown function 5 5 20% 3.2E+00
tnfn1_pw060418p01q155 FTN_0044 protein of unknown function 3 10% 3.5E+01
tnfn1_pw060328p02q177 FTN_1713 protein of unknown function 3 3 0% 7.0E-04
Transcription/Translation
tnfn1_pw060510p03q150 FTN_0949 rplI 50S ribosomal protein L9 2 75% 2.8E-05
tnfn1_pw060328p02q148 FTN_1393 transcriptional regulator, ArsR family 3 30% 2.3E-01
Transferase
tnfn1_pw060323p06q168 FTN_0545 glycosyl transferase, group 2 4 4 87% 2.7E-03
tnfn1_pw060328p01q142 FTN_0928 cysD sulfate adenylyltransferase subunit 2 3 3 57% 9.6E-02
tnfn1_pw060510p01q119 FTN_1428 wbtO transferase 6 2 50% 4.1E-01
tnfn1_pw060420p02q146 FTN_0300 glycosyl transferase, group 2 5 30% 6.8E-02
tnfn1_pw060323p03q121 FTN_0343 aminotransferase 2 7 20% 1.9E+01
tnfn1_pw060418p01q110 FTN_0200 UDP-3-O-[3-fatty acid] glucosamine N-acyltransferase 2 2 20% 2.4E+02
tnfn1_pw060510p01q103 FTN_0063 ilvE branched-chain amino acid aminotransferase protein (class IV) 5 3 13% 3.1E-01
tnfn1_pw060418p04q172 FTN_1418 manC mannose-1-phosphate guanylyltransferase 4 10% 8.3E-01
Transporter proteins
tnfn1_pw060420p01q180 FTN_0097 hydroxy/aromatic amino acid permease (HAAAP) family protein 4 4 89% 3.0E-05
tnfn1_pw060420p01q189 FTN_1611 - major facilitator superfamily (MFS) transport protein 5 7 89% 9.2E-02
tnfn1_pw060420p03q104 FTN_1593 oppA ABC-type oligopeptide transport system, periplasmic component 6 4 70% 8.4E-04
tnfn1_pw060323p06q117 FTN_1685 drug:H+ antiporter-1 (DHA1) family protein 4 70% 9.5E-02
tnfn1_pw060418p04q168 FTN_0141 ABC transporter, ATP-binding protein 6 5 70% 1.1E-01
tnfn1_pw060328p06q119 FTN_1549 drug:H+ antiporter-1 (DHA1) family protein 6 62% 1.3E-01
tnfn1_pw060510p03q140 FTN_0741 proton-dependent oligopeptide transporter (POT) family protein, di- or tripeptide:H+ symporter 3 60% 3.7E-02
tnfn1_pw060419p01q175 FTN_0984 ABC transporter, ATP-binding protein 2 56% 5.0E-01
tnfn1_pw060323p05q110 FTN_1215 kpsC capsule polysaccharide export protein KpsC 5 2 55% 8.7E-07
tnfn1_pw060323p01q175 FTN_1441 sugar porter (SP) family protein 4 4 50% 9.5E-05
tnfn1_pw060328p04q167 FTN_0997 proton-dependent oligopeptide transporter (POT) family protein, di- or tripeptide:H+ symporter 3 5 50% 2.4E-03
tnfn1_pw060419p01q170 FTN_1006 transporter-associated protein, HlyC/CorC family 2 50% 6.7E-01
tnfn1_pw060328p06q132 FTN_0728 predicted Co/Zn/Cd cation transporter 5 2 50% 2.1E+00
tnfn1_pw060328p08q167 FTN_0579 major facilitator superfamily (MFS) transport protein 3 44% 1.6E+00
tnfn1_pw060419p03q162 FTN_0115 Na+/H+ antiporter 4 4 40% 4.4E-02
tnfn1_pw060419p02q126 FTN_1581 small conductance mechanosensitive ion channel (MscS) family protein 3 3 40% 1.6E-01
tnfn1_pw060418p03q187 FTN_1733 - nicotinamide ribonucleoside (NR) uptake permease (PnuC) family protein 4 2 40% 3.4E+00
tnfn1_pw060418p02q182 FTN_1275 drug:H+ antiporter-1 (DHA2) family protein 4 40% 4.4E+00
tnfn1_pw060510p02q159 FTN_0688 galP2 galactose-proton symporter, major facilitator superfamily (MFS) transport protein 3 40% 2.2E+04
tnfn1_pw060323p07q172 FTN_1344 major facilitator superfamily (MFS) transport protein 4 4 37% 4.3E-05
tnfn1_pw060420p02q159 FTN_1716 kdpC potassium-transporting ATPase C chain 2 2 33% 2.0E+03
tnfn1_pw060419p01q133 FTN_1014 nicotinamide ribonucleoside (NR) uptake permease (PnuC) family protein 2 30% 3.8E+00
tnfn1_pw060510p02q118 FTN_1409 major facilitator superfamily (MFS) transport protein 2 20% 4.8E-01
tnfn1_pw060323p05q139 FTN_1166 metabolite:H+ symporter (MHS) family protein 1 20% 1.4E+00
tnfn1_pw060323p03q117 FTN_0005 corA divalent inorganic cation transporter 3 12% 6.9E+03
tnfn1_pw060328p03q163 FTN_0884 drug/metabolite transporter superfamily protein 4 4 11% 1.6E-03
tnfn1_pw060510p04q167 FTN_1681 fur ferric uptake regulation protein 2 10% 3.5E-01
tnfn1_pw060420p02q151 FTN_0018 sdaC serine permease 4 1 10% 9.9E+02
Type IV pili
tnfn1_pw060418p02q167 FTN_1137 pilQ Type IV pili secretin component 4 60% 1.5E+03