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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Neurochem. 2011 Jan;116(2):192–204. doi: 10.1111/j.1471-4159.2010.06824.x

Fig. 1.

Fig. 1

The interaction of F-actin with the components of Cdk5 complexes. The actin co-sedimentation assay was performed with F-actin polymerized from 5 μM G-actin in vitro. Recombinant GST (a), GST-p10 (b), p35 (c), p25 (d) and Cdk5 (e) were incubated with F-actin in the presence or absence of 10 nM actinin. After sedimentation at 100,000 × g for 30 min, both the supernatants (S) and the pellets (P) were fractionated by 12% SDS-PAGE and immunoblotted with anti-GST, anti-p35, anti-Cdk5, anti-β-actin, and anti-actinin antibodies.