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DNA substrates
| F-DNAa | 5′–CGCAGCCGTCCAACCAACTCAG GTCGATCCAATGCCGTCC–3′ |
| 3′–GCGTCGGCAGGTTGGTTGAGTCFCAGCTAGGTTACGGCAGG–5′ | |
| D-DNAb | 5′–CGCAGCCGTCCAACCAACTCA CGTCGATCCAATGCCGTCC–3′ |
| 3′–GCGTCGGCAGGTTGGTTGAGTXGCAGCTAGGTTACGGCAGG–5′ |
The ‘F’ in the 41mer template represents 2-aminopurine. The downstream 18-mer is 5′-phosphorylated.
The ‘X’ in the 41mer template represents dA for D-1 DNA and dT for D-7 DNA. The downstream 19-mer is 5′-phosphorylated.
