Table 5.
Kinetic parameters for non-natural nucleotide analog incorporation into single-nucleotide gap D-7 DNA catalyzed by WT human Pol λ at 37 °C.
| dNTP |
kp (s−1)a |
Kd (µM) |
kp/Kd (µM−1s−1) |
Kd Ratioa |
|---|---|---|---|---|
| dATPb | 1.5 ± 0.18 | 0.9 ± 0.3 | 1.7 | |
| dGTPb | (1.0 ± 0.2) × 10−2 | 8.4 ± 0.6 | 1.4 × 10−3 | 8 |
| dTTPb | (2 ± 1) × 10−4 | 7 ± 4 | 1.4 × 10−3 | 8 |
| dCTPb | (1.0 ± 0.2) × 10−2 | 7 ± 4 | 1.4 × 10−3 | 8 |
| dPTP | (3.3 ± 0.2) × 10−4 | 22 ± 2 | 1.5 × 10−5 | 24 |
| 1-dNaTP | (3.6 ± 0.3) × 10−4 | 11 ± 3 | 3.3 × 10−5 | 12 |
| 5-dNITP | (1.31 ± 0.04) × 10−2 | 1.5 ± 0.2 | 8.7 × 10−3 | 2 |
a
Calculated as (Kd )Analog/(Kd )dATP.
b
The kinetic parameters for natural dNTPs are from the work of Fiala et al.12