. Author manuscript; available in PMC: 2011 Nov 15.

Published in final edited form as: Bioorg Med Chem Lett. 2010 Sep 19;20(22):6394–6399. doi: 10.1016/j.bmcl.2010.09.088

Table 1.

Inhibition of hAMPK (α2, β1, γ1) by pyrazolopyrimidines


No.	R¹	R²	IC₅₀(µM)^a
1			7.7
2			3.5
3			1.8
4			0.28
5			0.06
6			0.27
7			0.14
8			0.22
9			2.1
10			2.2
11			1.1

values were measured using the Hot Spot™ filtration binding ³³P Kinase assay, Reaction Biology, Malvern, PA. Compounds were tested in ten-dose IC₅₀ mode with three-fold dilutions starting at 10 µM. All reactions were carried out in the presence of 10 µM ATP.