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. Author manuscript; available in PMC: 2010 Oct 20.
Published in final edited form as: Cold Spring Harb Protoc. 2010 Oct 1;2010:pdb.prot5511. doi: 10.1101/pdb.prot5511

RECIPES

MICROINJECTION BUFFER (10x)
Reagent Quantity (for 500 ml) Final concentration
KCl 1.86 g 50 mM
NaH2PO4 (anhydrous) 0.06 g 1 mM

Bring volume to 500 ml with sterile RNAse-free dH20. Adjust the pH to 7.2 and store at room temperature.