Table 1.

PCR amplification of promoters and terminators.

Component	Volume/reaction
10 × Platinum Pfx DNA Polymerase buffer	2.5 μl
dNTP (2.5 mM each)	2 μl
Primer mix (5 μM each)	2 μl
Yeast genomic DNA (~200 ng/μl)	1 μl
ddH2O	17.25 μl
Platinum Pfx DNA Polymerase (2.5 units/μl)	0.25 μl
Total	25 μl

PCR conditions: 94°C 4 min; 30 × (94°C for 30 sec, 55°C for 30 sec, 72°C for 45 sec); 72°C for 7 min; hold at 4°C.