Fig. 1.

Blood urea nitrogen, urinary rate, and kidney injury molecule-1 mRNA expression in wild-type and Nrf2-null mice after cisplatin treatment. A, blood urea nitrogen levels in wild-type and Nrf2-null mice 3 through 6 days after cisplatin (18 mg/kg i.p.) treatment (n = 4–15). B, blood urea nitrogen levels in wild-type and Nrf2-null mice 4 days after vehicle or cisplatin (18 or 25 mg/kg i.p.) treatment (n = 8–14). C, urine flow rate of control and cisplatin (18 mg/kg)-treated wild-type and Nrf2-null mice on day 4. Pooled urine volume was quantified from five to six mice per group in a metabolic cage for 6 h and normalized to body weight and time. D, messenger RNA expression of Kim-1 was quantified using total kidney RNA from control and cisplatin (18 mg/kg)-treated wild-type and Nrf2-null mice on day 4 (n = 4–5). Data are presented as means ± S.E. Messenger RNA data were normalized to wild-type control mice. Black bars represent wild-type mice, and gray bars represent Nrf2-null mice. * represents statistically significant differences (p < 0.05) compared with genotype control mice. † represents a statistically significant difference (p < 0.05) from cisplatin-treated wild-type mice.