Fig. 11.

Effect of CDDO-Im on cisplatin-induced nephrotoxicity in wild-type and Nrf2-null mice. A, wild-type and Nrf2-null mice were administered CDDO-Im (3 or 10 mg/kg per day p.o.) for 2 days, challenged with cisplatin (20 mg/kg i.p.), and evaluated 4 days later for changes in blood urea nitrogen. Data (n = 3–7) are presented as means ± S.E. Black bars represent wild-type mice, and gray bars represent Nrf2-null mice. * represents statistically significant differences (p < 0.05) compared with genotype control mice. † represents a statistically significant difference (p < 0.05) from cisplatin-treated wild-type mice. B, samples were fixed in zinc formalin prior to routine processing and paraffin embedding. Sections (5 μm) of kidneys were stained with hematoxylin and eosin and examined by light microscopy for the presence and severity of proximal tubule degeneration, apoptosis, and necrosis as well as renal cast formation and neutrophil infiltration.