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. 2010 Oct;335(1):2–12. doi: 10.1124/jpet.110.170084

Fig. 4.

Fig. 4.

Proliferation mRNA expression and immunohistochemical staining in kidneys of wild-type and Nrf2-null mice after cisplatin. A, PCNA staining (brown) in cisplatin (18 mg/kg)-treated wild-type and Nrf2-null kidney sections. Sections were counterstained with hematoxylin. Images were acquired at 40× magnification. B, PCNA staining was quantified in paraffin-embedded kidney sections (5 μm) from vehicle and cisplatin (18 and 25 mg/kg)-treated wild-type and Nrf2-null mice on day 4. PCNA-positive nuclei were quantified by counting three high-powered fields at 40× magnification. C, messenger RNA expression of PCNA, c-Myc, Ki67, and Topo2a was quantified using total kidney RNA from control and cisplatin (18 or 25 mg/kg)-treated wild-type and Nrf2-null mice on day 4. Data (n = 4–9) are presented as means ± S.E. Messenger RNA data are normalized to wild-type control mice. Black bars represent wild-type mice, and gray bars represent Nrf2-null mice. * represents statistically significant differences (p < 0.05) compared with genotype control mice. † represents a statistically significant difference (p < 0.05) from cisplatin-treated wild-type mice.