Fig. 5.

Basal expression of human PXR in the colon of hPXR mice. A, qPCR analysis of human PXR expression in colon and liver of hPXR mice and in colon of WT and Pxr-null mice. Mouse β-actin mRNA served as an internal control. B, Western blot analysis of human PXR expression in colon and liver of hPXR mice and in colon of WT and Pxr-null mice. Pooled nuclear protein (30 μg) was loaded for each sample (n = 3 per group). The monoclonal antibody against human PXR (H5017) specifically recognizes human PXR but not mouse PXR or other liver proteins. HNF4α was used as a loading control.