Fig. 6.

Eticlopride pretreatment attenuates METH-induced DAT complex formation (A and B) and loss of DAT monomer immunoreactivity (A and C), as assessed 7 days after METH treatment. Rats received four injections of METH (7.5 mg/kg s.c. per injection; 2-h intervals) or saline vehicle (1 ml/kg s.c. per injection; 2-h intervals) and were sacrificed 7 days later. Thirty minutes before each injection, rats were pretreated with eticlopride (0.5 mg/kg i.p.) or saline vehicle (1 ml/kg i.p.). A, a representative blot of saline/saline-treated (lane 1), saline/METH-treated (lane 2), eticlopride/saline-treated (lane 3), and eticlopride/METH-treated (lane 4) samples. Molecular mass (kDa) is shown adjacent to the representative blot. Columns represent the means and vertical lines indicate 1 S.E.M. determinations in six to eight rats. *, values different from saline-treated controls (p ≤ 0.05).