Fig. 3.

Caveolin-1 expression is increased in pregnant guinea pig. Western blot experiments (A) reveal increased expression of caveiolin-1 protein in myometrial samples from pregnant guinea pigs in early (15–34 days), mid (45–50 days), and late pregnancy (60–65 days) whether quantified as per protein loaded (B) or GAPDH expression (C). Intestine (GI) sampled from pregnant animals served as a positive comparative smooth muscle control and did not change as a result of pregnancy (not shown). Blots were imaged for the IR secondary antibody label in 680- and 800-nm channels as described, and data were recorded as relative intensity. Data are mean ± S.E.M. of determinations of pregnant tissue samples from 8 to 12 animals and nonpregnant controls (n = 3). *, p < 0.05; **, p < 0.01 compared with nonpregnant.