Fig. 4.

cGMP accumulation in the DIGs signaling domain in pregnant myometrium is agonist-specific. A, cGMP accumulation was measured in the soluble cell fraction of the myometrial tissue homogenate as stimulated by the NO donor SNAP (100 μM) in the presence or absence of the addition of ODQ (10 μM) or 2Cl-ATP (1 μM). B, cGMP measurements in the DIGs signaling domain fraction prepared on a density gradient were not stimulated by the soluble guanylyl cyclase activator (SNAP, 100 μM) but were significantly elevated by uGN (10 nM) and blocked by 2Cl-ATP (1 μM). SNAP had no effect in the DIGs fraction. Data are mean ± S.E.M., n = 6. ***, p < 0.001; ns, nonsignificant comparison.