Table 2.

Non-Nuclear Hyperacetylated Proteins Identified in Livers From Ethanol-Fed Rats

Spot	Protein	NCBI accession	App MW	Calc MW	Calc PI	Peptides matched	Sequence coverage (%)	MOWSE score	Mascot score	Expect value
a	Butyryl CoA synthetase 1		65	65	7.6	16	44	1.32 × 1011	123	3.4 × 10−8
		GI:197245828
b	1-pyrroline-5-carboxylate dehydrogenase	GI:149024431	67	62	8.3	12	32.6	1.05 × 107	70	6.4 × 10−3
c	Keratin contamination
d	Aconitate hydratase	GI:40538860	93	85	7.9	27	40	6.21 × 1014	170	6.9 × 10−13
e	ATP synthase β subunit	GI:54792127	55	56	5.1	37	69.6	1.72 × 1018	267	1.1 × 10−22
f	4-trimethylamino-butyraldehyde dehydrogenase	GI:149058126	58	56	6.9	19	46.3	6.87 × 109	147	1.4 × 10−10
g	Succinate-semialdehyde dehydrogenase	GI:182676407	53	56	8.4	19	46.1	3.06 × 1010	121	5.4 × 10−8
h	Ethanolamine-phosphate cytidylyltransferase	GI:50925459	50	43	6.4	17	45.9	9.01 ×109	117	1.4 ×10−7
i	Adenosine kinase	GI:149031258	43	38	5.8	15	43.8	7.18 × 106	101	5.4 × 10−6
	β-Actin	GI:4501885	43	42	5.3	9	28.8	4.07 × 104	43	3.4
j	Fructose-1,6-bisphosphatase 1	GI:51036635	38	40	5.9	14	47.9	5.01 × 108	145	2.2 × 10−10
k	δ-Aminolevulinic acid dehydratase	GI:6978483	36	36	6.3	17	54.5	5.80 × 108	126	1.7 × 10−8
	Transaldolase	GI:149061610	36	36	8.2	11	25.3	2.57 × 104	74	3.1 × 10−3
l	α-methylacyl-CoA racemase	GI:6981184	41	40	6.2	11	39.3	1.39 × 106	89	9.0 × 10−5
m	3-oxo-5-β-steroid-4-dehydrogenase	GI:20302063	36	37	6.2	14	45.4	4.65 × 109	62	3.9 × 10−2
	Transaldolase	GI:149061610	36	36	8.2	15	29.4	1.12 × 106	55	2.1 × 10−1
	Ornithine transcarbamylase	GI:6981312	36	36	8.2	10	45.2	1.38 × 106	45	2.1
n	Dihydropicolinate synthase	GI:157822207	32	34	8.5	12	55.5	3.48 × 105	66	1.7 × 10−2
o	Trans-2-enoyl-CoA reductase	GI:8393848	36	40	8.9	7	23.3	5.31 × 103
	Fructose-bisphosphate aldolase B	GI:158081751	36	40	8.7	15	50.8	1.03 × 107	92	3.9 × 10−5
p	Fructose-bisphosphate aldolase B	GI:158081751	38	40	8.7	8	30.1	1.92 × 104	72	4.9 × 10−3
	Peroxisomal δ 3, δ 2-enoyl CoA isomerase	GI:55741520	38	43	9.1	11	31.7	2.05 × 107	75	2.1 × 10−3
	Peroxisomal δ3, δ 2-enoyl CoA isomerase	GI:55741520	38	43	9.1	11	31.7	2.05 × 107	75	2.1 × 10−3
q	Glutathione peroxidase 1	GI:2654236	20	22	7.7	9	63.2	3.29 × 105	125	2.2 × 10−8
r	Glutathione S-transferase μ2	GI:62653546	27	26	6.9	16	55.0	1.32 × 109	143	3.4 × 10−10
s	Superoxide dismutase	GI:8394331	24	25	9.0	5	33.3	9.07 × 103	58	1.1 × 10−1
t	60 kDa heat shock protein	GI:1334284	63	58	5.3	30	57.8	5.60 × 1017	220	6.9 × 10−18
u	60 kDa heat shock protein	GI:1334284	63	58	5.3	31	60	1.90 × 1018	219	8.6 × 10−18
v	Isovaleryl-CoA dehydrogenase	GI:6981112	42	43	8.0	17	43.9	7.81 × 1010	105	2.2 × 10−6
w	Glutamate dehydrogenase 1	GI:6980956	55	61	8.1	17	39.6	2.49 × 108	119	8.6 × 10−8
x	Glutamate dehydrogenase 1	GI:6980956	55	61	8.1	25	50.0	3.78 × 1012	186	1.7 × 10−14
y	Glutamate dehydrogenase 1	GI:6980956	55	61	8.1	22	48.9	2.41 × 1012	170	6.9 × 10−13
z	Glutamate dehydrogenase 1	GI:6980956	55	61	8.1	27	51.1	1.75 × 1015	205	2.2 × 10−16
aa	4-hydroxyphenyl pyruvate dioxygenase	GI:8393557	42	45	6.3	13	42.2	5.52 × 108	94	2.7 × 10−5
	Acyl-CoA thioesterase 2	GI:48675862	42	50	8.2	7	31.8	2.62 × 105	45	2.0
bb	Acyl-CoA thioesterase 2	GI:48675862	42	50	8.2	13	39.5	2.76 × 105	86	1.6 × 10−4
cc	Acyl-CoA thioesterase 2	GI:48675862	42	50	8.2	9	33.8	2.18 × 104	60	6.2 × 10−2
	3-ketoacyl-CoA thiolase	GI:149027156	42	50	8.2	13	46.3	8.50 × 108	77	1.3 × 10−3
dd	3-ketoacyl-CoA thiolase	GI:149027156	42	50	8.2	9	35.6	1.47 × 107	60	6.2 × 10−2
	Argininosuccinate synthase	GI:25453414	42	46	7.6	13	41.5	2.67 × 109	75	2.4 × 10−3
ee	Cystathionine γ-lyase	GI:13699175	41	54	7.5	13	51.3	6.29 × 107	82	4.5 × 10−4
	Acetyl-CoA acetyltransferase	GI:135757	41	42	8.4	11	30.7	2.06 × 107	40	6.2
	Medium-chain specific acyl-CoA dehydrogenase	GI:8392833	41	47	8.6	11	28.0	2.94 × 106	64	3.1 × 10−2
ff	26S protease regulatory Subunit S10 B	GI:81294202	41	43	7.2	22	56.7	5.65 × 1012	113	3.4 × 10−7
gg	β-Ureidopropionase	GI:16758704	41	44	6.5	9	33.8	5.16 × 105	45	2.3
	Glutamine synthetase	GI:142349612	41	42	6.6	11	33.5	2.29 × 107	49	8.4 × 10−1
	Medium-chain specific acyl-CoA dehydrogenase	GI:8392833	41	47	8.6	13	39.9	3.75 × 106	75	2.0 × 10−3
hh	Enoyl-CoA hydratase	GI:17530977	30	32	8.4	15	50.0	4.52 × 106	120	6.9 × 10−8
ii	Enoyl-CoA hydratase	GI:17530977	30	32	8.4	12	44.1	3.76 × 104	88	1.1 × 10−4
	Electron transfer flavoprotein subunit β	GI:51948412	30	28	7.6	6	25.5	2.84 × 104	28	1.1 × 102
jj	Hydroxyacyl-CoA dehydrogenase	GI:17105336	32	34	8.8	15	53.2	3.07 × 104	88	9.9 × 10−5
	Hydroxymethylglutaryl-CoA lyase	GI:13242293	32	34	8.7	11	39.4	1.60 × 107	58	1.1 × 10−1
kk	2,4-dienoyl-CoA reductase	GI:67476443	32	36	9.1	15	38.5	2.47 × 1010	104	2.7 × 10−6