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. Author manuscript; available in PMC: 2010 Oct 20.
Published in final edited form as: Traffic. 2009 Apr 29;10(8):1006–1018. doi: 10.1111/j.1600-0854.2009.00936.x

Figure 2.

Figure 2

ALP is inefficiently transported to the Golgi complex in erv26Δ cells. A) Wild-type (CBY2151) and erv26Δ (CBY2240) strains expressing full-length pro-ALP (pFL8) were monitored in pulse-chase experiments as described under Materials and Methods. Primary IPs were performed with anti-ALP or anti-CPY serums followed by secondary IPs to measure α1,6-mannose modified carbohydrate. Positions of pro-ALP (p), mature ALP (m) and a soluble breakdown product (*) are indicated as are the ER (p1), Golgi (p2) and mature (m) forms of CPY. B) Graphical representation of the results in (A) showing α1,6-mannose modified target protein divided by total target protein at each time-point.