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. Author manuscript; available in PMC: 2010 Oct 20.
Published in final edited form as: Traffic. 2009 Apr 29;10(8):1006–1018. doi: 10.1111/j.1600-0854.2009.00936.x

Figure 6.

Figure 6

The membrane-bound lumenal domain of ALP is sufficient to specify Erv26p-dependent export. A) Wild-type (CBY2151) and erv26Δ (CBY2240) cells expressing full-length pro-ALP (pFL8) or the Och1CT-ALP construct (pCIP2) were converted to spheroplasts and proteins detected by immunoblot. The pro-ALP (p), mature ALP (m) and a soluble breakdown product (*) are indicated. B) Extracts from the wild-type and erv26Δ cells expressing Och1CT-ALP were fractionated on sucrose gradients and proteins detected by immunoblot. C) Pulse-chase analysis of Och1CT-ALP in wild-type and erv26Δ cells expressing pCIP2. D) Graphical representation of the results in (C) plotting α1,6-mannose modified Och1CT-ALP protein divided by total Och1CT-ALP.