Table 1.

Effects of vehicle or 17 β-estradiol (E₂) pretreatment on hOB cell conversion of 1-[¹⁴C]arachidonic acid into prostaglandins^a

Treatment	PGE2, %b	6-keto-PGFb1α, %
Control	2.8 ± 0.3	1.9 ± 0.2
Control + E2	2.4 ± 0.4	1.5 ± 0.3
TGFβ	10.3 ± 2.3	2.8 ± 0.2
TGFβ + E2	9.1 ± 1.6	2.6 ± 0.3
TNF	10.9 ± 2.3	2.7 ± 0.4
TNF + E 2	9.6 ± 1.5	2.5 ± 0.4
TGFβ + TNF	27.6 ± 2.4	2.7 ± 0.4
TGFβ + TNF + E2	28.6 ± 2.9	2.7 ± 0.4

^ahOB cells were incubated for 48 hours with vehicle (0.1% ethanol) or with 17 β-estradiol (E₂ ), and then stimulated with the cytokines, as indicated, for 24 hours. The conditioned media were collected and individual prostaglandins were separated by TLC.

^bData are calculated as the % cpm of individual prostaglandin bands vs. the total cpm of the scraped and scintillation-counted TLC lane. Each of the cytokine treatments stimulated product (PGE₂, 6-keto-PGF_1α) formation, irrespective of 17 β-E₂ pretreatment (p ≤ 0.05)