Figure 1.

Male-Enlarged Regions Are Genotype Specific and Colocalize with fruitless Expression

(A) Schematic of Drosophila neuropil compartments and olfactory system. Projection neurons are cyan, mushroom body neurons are green.

(B) Surface rendering of male (green)- and female (magenta)-enlarged regions on our template brain.

(C) Summary of main male-enlarged regions (MERs). Green: pars intercerebralis; red: arch; mustard: ring; turquoise: lateral junction; light blue: DA1 glomerulus; yellow: tips of mushroom body gamma lobes.

(D–K) Two individual slices from average template brains for control male (D and H), control female (E and I), tra mutant female (F and J), and fru^M mutant female (G and K). In (D)–(G), arrow indicates male-enlarged arch; in (H)–(K), it shows the male expansion of the ring neuropil.

(L) Overlap between MERs (blue) and fru^Gal4 expression pattern (green).

(M and N) Z projections (standard deviation) of the fru^Gal4 driving CD8-GFP in males (M) and females (N). For clarity, cell bodies in the surface of the brain and the mushroom bodies have been removed. A false color lookup table has been applied to emphasize contrast.

(O) Schematic of Drosophila sex determination hierarchy. Bold-type gene products are active, gray are inactive.

(P) Quantification of the volume of the MER for control and mutant flies. The central line indicates the median, the box represents the 25% and 75% quartiles, and the whiskers extend to ±1.5 times the interquartile range.

Scale bar in all panels represents 50 μm. See also Figure S1.