Figure 5.

fru+ Lateral Horn Neurons

(A) Cluster analysis of lateral horn innervation patterns of many examples of five classes of fru+ lateral horn neuron (LHN). Color bars indicate class and sex, with five hues (blue, red, orange, magenta, and green) representing male clones and matched but paler colors representing female clones. For four of these classes, clones of the same sex cocluster but clones of the same class from different sexes do not. The exception is aIP-b, in which male and female samples do not separate (light and dark blue). See Supplemental Experimental Procedures for details.

(B) Volume rendering of seven classes of fru+ LHNs in males and females. Neuronal arbors are colored based on UAS:synaptotagmin staining and neuronal morphology. Cell bodies are in red, axons are in green, dendrites are in blue, unclassified neurites are in black. We did not obtain UAS:synaptotagmin data for clone aSP-a or the male aSP-h clone.

(C) The bundle of aDT-a axons (red) traversing the LH serves as a simple landmark to separate pheromone and general odor zones of the lateral horn (dotted white line) [7].

(D1 and D2) Processes of all male (D1) and female (D2) fru+ LHNs (magenta) are restricted to the pheromone zone. Only LHN neurites within the LH are shown. Scale bar represents 50 μm. See also Figure S3.