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. 2010 Sep 14;1(3):99–112. doi: 10.1159/000319859

Fig. 7.

Fig. 7

CCDC88C expression studies by quantitative RT-PCR in cDNA from various human fetal and adult tissues showed highest relative expression in fetal brain and highest relative expression in adult pancreas (A). B Upper part represents a scheme of the various isoforms and the lower part shows the results of isoform-specific CCDC88C RT-PCR on fetal and adult brain cDNA panels. Size standard is puC Mix 8 (Fermentas) and GAPDH primers were used as positive control. IF1 is expressed in all fetal brain tissues investigated, but only in adult cortex and medulla. From the other isoforms only IF3b was found expressed in brain tissues: in fetal cerebellum and pons and in adult medulla. Primers for isoform specific RT-PCR were located in exon 14/15 and exon 15 for IF1 (617 bp), exon 25b and exon 29 for IF2 (488 bp), in exon 26b and 27b for IF3a (403 bp), in exon 26 and 27b for IF3f3ar (234 bp), in exon 26b and exon 28 for IF3b (540 bp) and in exon 2 and 3b for IF4 (540 bp).